CnT-05 is a 10% serum medium that allows efficient isolation and extended in vitro culture of human, mouse, and canine fibroblasts.

It uses an optimsed basal medium with additional trace elements, protective antioxidants and vitamins. The basal medium is then supplemented with 10% FCS just prior to use.

It can easily be upgraded for clinical applications by replacing the FCS with human serum. Contact us at scientist@cellntec.com for further details and pricing.

For increased isolation efficiency and growth rate, it is now recommended to switch to the CnT-PR-F Fibroblast medium.

The medium undergo a range of QC tests in our lab before being released for sale. Please see the datasheet for details.

Thawing, seeding and passaging protocols are particularly important for optimal cell growth. Please visit our Protocols Page for our recommendations.

Please visit our publications database and search by tissue type to obtain a range of articles that have used CELLnTEC primary cells and media in this specific field.

Printable datasheet

Fibroblast Medium

CnT-05 is a liquid culture medium kit including both basal medium and supplements. CnT-05 contains serum. It does not contain antibiotics / antimycotics. The basal medium contains amino acids, minerals, vitamins and organic compounds, but is protein free.

Formulated for human and mouse fibroblasts (i.e. dermal, vaginal, airway). May be used for other species as well (not yet tested).
Tissue type
Pack size
CnT-05 is a 500 mL kit consisting of:
  • CnT-BM.2: 500 mL CnT Basal Medium 2 (liquid)
  • CnT-05.S: containing two supplements (Supplements A and B; delivered frozen; to be added to 500 mL basal medium prior to use) 
Product use
Developed for improved fibroblast isolation and growth.
Media Type
Culture condition
This medium is designed for use in a 5% CO2 atmosphere. For suggested cell culture protocols, please see our resources section.
For routine cell cultivation, CELLnTEC recommends to work without antibiotics / antimycotics.
Storage / Shelf life
  • CnT-BM.2 basal medium: Store at 4°C in the dark. For expiry date, please see label on bottle. Please do not freeze.
  • CnT-05.S supplements: Store at -20°C. For expiry date, please see label on tube and in bag.
  • The complete medium (basal medium supplemented with the corresponding supplements) must be stored at 4°C in the dark. Storage in this manner will provide a minimum shelf life of 8 weeks. Please do not freeze.
Important: The quality of cell culture media is rapidly degraded by light. Store in the dark, and minimize all light exposure during handling.
Quality control
Media functionality and cell morphology tested by cell cultivation over 2 passages in supplemented medium. Osmolality, pH and concentration of various ions are tested. Free of bacteria, fungi and mycoplasma contamination.
Shipping condition
  • CnT-BM.2 basal medium is shipped at ambient temperature.
  • CnT-05.S supplements are shipped frozen.
Intended use
For research use only. Not for use in therapy or diagnostics.
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