The VitroAge medium (CnT-AG2) is specifically designed to age epidermal keratinocytes over several passages, without the need for articial pro-aging treatments such as perioxide.
Traditional culture media have evolved over 50 years to provide strong proliferation for extended periods. They contain many protective and stimulatory factors and nutrients, that exert a powerful anti-aging effect.
CnT-AG2 lacks these protective elements. Keratinocytes age naturally in this medium over a period of several weeks, without the need for acute doses of oxidizers or other synthetic pro-aging chemicals.
The Vitroage epidermal model maintains normal cell morphology, but generates several well documented in vivo signs of ageing including reduced lifespan and disrupted metabolism. For an overview of key ageing-related literature, please click here.
It is now well documented that the aged epidermis is characterized by a reduced number and function of transient-amplifying progenitor cells (Charruyer et al, JID 2009). In particular, these progenitors were found to cycle more slowly, and fewer times in aged tissue. A similar reduction in cell cycle kinetics is also observed in VitroAge during 4 weeks of culture (see Fig at left).
Keratinocytes aged in VitroAge medium demonstrate a range of other age-related changes. Please click here to view a poster with additional details.
There are several important protocol considerations when culturing keratinocytes in CnT-AG2 medium. Please visit our protocols page for more info.