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CnT-DNP-10

  • Isolation
  • Expansion
  • Differentiation
  • Homeostasis
  • Cryopreservation
  • Co-culture
  • Aging
  • Staining

CnT Dispase II, Neutral Protease

An optimal emzyme for isolation of primary epithelial cells.

Catalog CnT-DNP-10
Content 5 x 100 mg (approx) powder aliquots (separate tubes)

HIGHLIGHTS:

  • Gentle but effective separation of epithelial and stromal cells, allowing populations of high purity to be seeded into the primary culture

Description

Dispase® is a neutral protease that cleaves fibronectin, collagen IV, and to lesser extent collagen I, and can be used to separate epithelial tissues from underlying mesenchyme.

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Specifications

Application
Isolation
Free of Human & Animal Component
Yes
Chemically defined
No
Clinically upgradable
No
Volume
5 x 100 mg
Quality level
Research Grade

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Scientific resources

Dispase II (Neutral Protease) is an optimal enzyme for the isolation of primary epithelial cells.

It provides gentle but effective separation of epithelial and stromal cells, allowing populations of high purity to be seeded into the primary culture.

It cleaves fibronectin, collagen IV, and to lesser extent collagen I and can be used to separate epithelial tissues from underlying mesenchyme.

Our dispase product CnT-DNP-10 is provided as 5 x 100 mg (approx) powder aliquots. Powder amounts may vary from lot-to-lot, due to differing activities.

Each 100 mg aliquot is sufficient to make up 40 mL of 1x solution, which is sufficient for 2-4 isolations (depending on the quantity of tissue used). The final 1x concentration after solubilization will be 2.4 U/mL.

With 5 powder aliquots in total, CnT-DNP-10 is thus sufficient for up to 20 isolations.

For recommended isolation protocols, please see the Isolation Protocols page.


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Scientific Literature

Title Authors Year Tissue type
Protective roles of tRNA-derived small RNA tRF-Ile-AAT-019 in pathological progression of psoriasis Jinrong Zeng, Yajie Xie, Hanyi Zhang, Yuezhong Zhang, Yue Zhang, Liyao Liu, Qian Hu, Lu Zhou, Lihua Gao, Wenbin Tan, Zhibing Fu, Jianyun Lu 2022 Epidermal
Spatiotemporal dynamics of PIEZO1 localization controls keratinocyte migration during wound healing Jesse R Holt, Wei-Zheng Zeng, Elizabeth L Evans, Seung-Hyun Woo, Shang Ma, Hamid Abuwarda, Meaghan Loud, Ardem Patapoutian, Medha M Pathak 2021 Epidermal
SUV39H2 epigenetic silencing controls fate conversion of epidermal stem and progenitor cells Pierre Balmer, William V.J. Hariton, Beyza S. Sayar, Vidhya Jagannathan, Arnaud Galichet, Tosso Leeb, Petra Roosje, Eliane J. Müller 2022 Epidermal
Title Year
CELLnTEC Catalog 2023 Download

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