CELLnTEC

CnT-MSCDIFF-CHOND.S

Features

Click for enlarged image. Strong alcian blue staining of BM-MSC pellet differentiated down the chondrogenic lineage after 4 passages of expansion in CnT-PR-MSC-XF medium. Control medium: high glucose DMEM + 10% FCS + differentiation supplements. Images courtesy of Institute of Surgical Technology and Biomechanics, University of Bern.

The #CnT-MSCDIFF-CHOND.S chondrogenic differentiation supplement enables high efficiency differentiation of MSCs maintained in one of the CnT-Prime family of MSC expansion media.

For optimal chondrogenic differentiation, please follow the recommended protocol. It is available for download in the Protocols Section of this website.

Printable datasheet

Chondrogenic Differentiation Supplement

The #CnT-MSCDIFF-CHOND.S chondrogenic differentiation supplement is used to differentiate MSCs maintained in one of the CnT-Prime family of MSC expansion media.

Species
Tested with human cells, may be used with other species as well.
Tissue type
MSC
Pack size
2 x 1 mL
Product use
The supplement is added to 50 mL of expansion medium, and the cells then cultured according to the chondrogenic differentiation protocol. See the Protocols Page for culture protocols.
Media Type
2D-Diff
Defined
Yes
ACF
Yes
Culture condition
This medium is designed for use in a 5% CO2 atmosphere. For suggested seeding and culture protocols, please visit the resources section of www.cellntec.com
Note
CELLnTEC does not recommend the use of antibiotics / antimycotics during cell differentiation.
Storage / Shelf life
Store frozen below -15oC. For best before date, see label. Thaw supplements at room temperature, ensure contents do not rise above 12oC at the end of the thawing process. Supplemented medium has a remaining shelf-life of 4-weeks when stored at 4oC in the dark. Certain culture media components are very sensitive to light. Minimize light exposure at all times.
Quality control
Supplement functionality is tested by evaluating differentiation primary human MSC down the chondrogenic lineage according to the recommended protocol. Free of bacteria, fungi and mycoplasma contamination.
Shipping condition
Supplement is shipped frozen.
Intended use
For research use only. Not for use in therapy or diagnostics.
Last update
2017-09-27