CELLnTEC

Features

CnT-PR-EF is a chemically defined, animal component free medium for for differentiation of human iPSC and ESC down corneal lineages.

For detailed instructions of how CnT-PR-EF an be supplemented to induce iPSC to differentiate into corneal epithelium, please see Nature Protocols (Hayashi et al. 2017).

This medium can also be upgraded for clinical applications.

Like to try a test sample? Click here for more information.

Printable datasheet

iPSC Corneal Differentiation Medium

CnT-PR-EF is a liquid culture medium kit, that is provided fully supplemented, ready to use. It does not contain antibiotics / antimycotics, and is free of EGF and FGF. It is a customized product, that is produced to order.

Tissue type
IPSC
Pack size
500 mL bottle, supplemented with growth factors, but EGF and FGF-free.
Product use
Differentiation of human ESC and iPSC down a corneal lineage
Media Type
2D-Diff
Defined
Yes
ACF
Yes
Culture condition
This medium is designed for use in a 5% CO2 atmosphere. For suggested cell culture protocols, please see our resources section.
Note
For routine cell cultivation, CELLnTEC recommends to work without antibiotics / antimycotics.
Storage / Shelf life
Store frozen below -15oC. For best before date, see label. To prepare medium for use, it is preferable to thaw overnight at 4oC. Alternatively, medium may be thawed at room temperature for approx 5-6 hours. If thawing at room temperature, ensure contents do not rise above 15oC at the end of the thawing process. Do not thaw in a hot water bath. Once thawed, medium has a remaining shelf-life of 6-weeks when stored at 4oC in the dark. Certain culture media components are very sensitive to light. Minimize light exposure at all times.
Quality control
Osmolality, pH and concentration of various ions are tested. Free of bacteria, fungi and mycoplasma contamination.
Shipping condition
Medium is shipped frozen.
Intended use
For research use only. Not for use in therapy or diagnostics. For further information about the clinical use of this product, please contact scientist@cellntec.com
Last update
2017-12-04