CELLnTEC

Features

Isolation Efficiency

CnT-PR-F delivers 50% more cells on day 5 post isolation compared with traditional 10% serum media.

CnT-Prime Fibroblast is a 1% serum medium that delivers improved isolation efficiency and proliferation rate of human and animal fibroblasts in comparison with traditional 10% serum media. It was developed using human tissue, but may also be used with other species (e.g. mouse).

The reduction to a 1% serum medium is achieved through the addition of a range of fully defined growth factors and co-factors to improve their binding to cell surface receptors.

It can easily be upgraded for clinical applications by replacing the FCS with human serum. Contact us at scientist@cellntec.com for further details and pricing.

By reducing the dependence on serum, CnT-Prime Fibroblast is significantly more consistent than traditional high-serum formulations.

ProlifRate
Figure: Growth rate of primary human dermal fibroblasts in the 24 days (5 passages) following isolation is 40% faster in CnT-PR-F than in the traditional CnT-05 (10% serum) medium. Cells were grown on standard tissue culture plastic, passaged with accutase, and seeded at 1’000 cells per sq. cm.

Test samples (#CnT-PR-F-SAMPLE) are now available for testing.

For induction of other cell behaviours such as ECM secretion, the CnT-PR-ECM medium is recommended.

Printable datasheet

CnT-Prime Fibroblast Medium

CnT-Prime Fibroblast Medium is a highly refined medium for fibroblast culture. It uses an advanced basal medium supplemented with a range of growth factors, thereby reducing the serum requirement down to 1%.

Species
May be used for human, mouse and rat tissue.
Tissue type
Skin
Pack size
500 mL bottle, fully supplemented with growth factors. No further additions required.
Product use
Developed specifically for optimal isolation efficiency and proliferation of fibroblasts. Recommended seeding density immediately after isolation: 12'000 cells per sq. cm. Recommended seeding density after passaging: 1'000 cells per sq. cm. See the Protocols Page for details.
Media Type
2D-Prolif
Defined
No
Culture condition
This medium is designed for use in a 5% CO2 atmosphere. For suggested seeding and culture protocols, please visit the resources section of www.cellntec.com
Note
For routine cell cultivation, CELLnTEC does not recommend the use of antibiotics / antimycotics.
Storage / Shelf life
Store frozen below -15oC. For best before date, see label. To prepare medium for use, it is preferable to thaw overnight at 4oC. Alternatively, medium may be thawed at room temperature for approx 5-6 hours. If thawing at room temperature, ensure contents do not rise above 12oC at the end of the thawing process. Once thawed, medium has a remaining shelf-life of 6-weeks when stored at 4oC in the dark. Certain culture media components are very sensitive to light. Minimize light exposure at all times.
Quality control
Media composition is tested via osmolality, pH and the concentration of various ions. Media functionality is tested by evaluating growth and morphology or primary human fibroblasts over at least 2 passags. Free of bacteria, fungi and mycoplasma contamination.
Shipping condition
Medium is shipped frozen.
Intended use
For research use only. Not for use in therapy or diagnostics.
Last update
2016-11-21