The reduction to a 1% serum medium is achieved through the addition of a range of fully defined growth factors and co-factors to improve their binding to cell surface receptors.
It can easily be upgraded for clinical applications by replacing the FCS with human serum. Contact us at email@example.com for further details and pricing.
By reducing the dependence on serum, CnT-Prime Fibroblast is significantly more consistent than traditional high-serum formulations.
Figure: Growth rate of primary human dermal fibroblasts in the 24 days (5 passages) following isolation is 40% faster in CnT-PR-F than in the traditional CnT-05 (10% serum) medium. Cells were grown on standard tissue culture plastic, passaged with accutase, and seeded at 1’000 cells per sq. cm.
Test samples (#CnT-PR-F-SAMPLE) are now available for testing.
For induction of other cell behaviours such as ECM secretion, the CnT-PR-ECM medium is recommended.