CELLnTEC

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vitc_responsiveness

Dermal fibroblasts growing in CnT-PR-FH homeostasis medium show 5x greater responsiveness to the treatment with Vitamin C than control cells growing in standard proliferation medium.

The CnT-PR-FH medium is designed for maintenance of primary human dermal fibroblasts in a homeostatic state with only minimal growth factor stimulii, sufficient to maintain their function for up to a week in culture. It is a fully defined medium.

It can easily be upgraded for clinical applications. Contact us at scientist@cellntec.com for further details and pricing.

In the absence of the strong growth factor stimulation found in standard proliferation media, fibroblasts growing in CnT-PR-FH show heighted responsiveness to experimental stimulii (for example Vitamin C to stimulate collagen secretion, as shown at left).

Fibroblast responsiveness to vitamin C treatment (100 ug/mL) is 5x higher than in the standard CnT-PR-F proliferation medium. Confluent dermal fibroblasts were switched to the respective media at confluence, and grown for a further 5 days. Total collagen was then lysed in the well (acetic acid), quantified using sirius red, and normalised to gDNA to correct for any differences in cell number.

It is strongly recommended to evaluate total collagen using in-well lysis of all the cells and deposited ECM (as opposed to evaluation of only the supernatant), and to normalise for cell number using gDNA.

A complete protocol is available for download.

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Printable datasheet

CnT-Prime Fibroblast Homeostasis Medium

CnT-PR-FH is a fully supplemented, frozen, and ready to use culture medium, designed for maintenance of primary fibroblasts in a homeostatic state for up to one week. It does not contain antibiotics / antimycotics.

Species
Developed for human fibroblasts. May be used for other species as well.
Tissue type
Skin
Pack size
250 mL bottle, fully supplemented with growth factors. No further additions required.
Product use
Developed for maintenance of primary fibroblasts in a homoestatic state for up to 1 week of culture.
Media Type
2D-Prolif
Defined
Yes
Culture condition
This medium is designed for use in a 5% CO2 atmosphere. For suggested cell culture protocols, please see our resources section.
Note
For routine cell cultivation, CELLnTEC recommends to work without antibiotics / antimycotics.
Storage / Shelf life
Store frozen below -15oC. For best before date, see label. To prepare medium for use, it is preferable to thaw overnight at 4oC. Alternatively, medium may be thawed at room temperature for approx 5-6 hours. If thawing at room temperature, ensure contents do not rise above 15oC at the end of the thawing process. Do not thaw in a hot water bath. Once thawed, medium has a remaining shelf-life of 6-weeks when stored at 4oC in the dark. Certain culture media components are very sensitive to light. Minimize light exposure at all times.
Quality control
Media functionality tested by cell cultivation with primary human fibroblasts. Osmolality, pH and concentration of various ions are tested. Free of bacteria, fungi and mycoplasma contamination.
Shipping condition
CnT-PR-FH is shipped frozen.
Intended use
For research use only. Not for use in therapy or diagnostics.
Last update
2016-11-21