Next Generation Expansion of adMSCs
The chemically defined CnT-Prime MSC-CD medium is the first MSC expansion medium that is free of all human and animal-derived components. In addition, it does not require the use of plate coatings, and does not contain any serum or blood cell-derived proteins.
It can easily be upgraded for clinical applications. Contact us at firstname.lastname@example.org for further details and pricing.
It uses exclusively recombinant growth factors to provide balanced cell expansion without
overstimulation, thus improving retention of key markers over multiple passages in comparison with serum-containing media.
As a result of its fully defined, human- and animal-component free formulation, adipose-derived MSC growing in CnT-Prime MSC-CD medium are ideally suited for clinical use. For highest isolation efficiency, the xeno-free (Prime MSC-XF) medium is recommended.
The cell population is CD45-/CD31-/CD73+/CD90+/CD105+/CD34+, and importantly at least 20% of the cell population remains CD34-positive at passage 4.
Figure: Strong CD34 expression in CnT-PR-MSC-CD medium, passage 4.
Figure: High colony forming efficieny of adMSC maintained in CnT-PR-MSC-CD medium for 4 passages (CFE assay performed with CnT-PR-MSC-XF medium according to the recommended protocol).
Cells may at any time be induced to differentiate following addition of the necessary differentiation supplements. Please visit the MSC homepage for more information about other CnT-Prime MSC products.
Please view the Culture Notes document for specific recommendations regarding the use of the CnT-Prime MSC-CD medium.
To download a flyer summarizing the CnT-Prime MSC-CD medium, please click here.
The CnT-PR-MSC-CD medium is currently in the beta testing phase. Please contact email@example.com to request your test sample.