CELLnTEC

CnT-PR-MSC-XF

Features

CnT-PR-MSC-XF medium provides faster expansion of bone marrow-derived MSC than widely used alternatives such as αMEM + 10% FCS.

The new CnT-Prime MSC-XF medium enables high efficiency isolation and expansion of both adipose and bone marrow derived MSCs, without plate coating, in a fully xeno-free environment that can be upgraded for clinical use!

MSC_XF Medium
Figure: BM-MSC (P4) and AD-MSC (P6) growing in CnT-PR-MSC-XF medium.

Cells growing in CnT-Prime MSC-XF medium also demonstrate extended longevity, and remain strongly multipotent for at least 4 passages.

Cells can be induced to differentiate down adipogenic, osetogenic or chondrogenic lineages following addition of the corresponding differentiation supplement.

Adipogenesis MSC-XF
Figure: Adipogenesis in BM-MSC (P4). Cells growing in CnT-PR-MSC-XF medium remain strongly multipotent for extended periods. Images courtesy of Institute of Surgical Technology and Biomechanics, University of Bern.

For an overview of the performance of bone marrow-derived MSC in CnT-PR-XF medium, please download this A4 Flyer.

Adipose-derived MSC expanded in CnT-PR-MSC-XF are CD45-/CD31-/CD73+/CD90+/CD105+/CD34+/-, and importantly at least 8% of the cell population remains CD34-positive at passage 4.
cd34_cnt-pr-msc-xf
Figure: CD34 expression in AD-MSC, CnT-PR-MSC-XF medium, passage 4. Analysis courtesy of Dr Sean Hall, Dept Clinical Research, University of Bern.

AD-MSC expanded in CnT-Prime MSC-XF medium also maintain strong multipotency for extended periods, with class leading differentiation efficiency after 4 passages in culture.

msc_p4_lipids_cd_sct-acf
Figure: CnT-Prime MSC Media demonstrate high adipogenic differentiation efficiency compared with leading competitors after 4 passages in culture. (A) CnT-PR-MSC-CD, (B) CnT-PR-MSC-XF, (C) Mesencult-ACF

cfe_xf_p4
Figure: High colony forming efficiency of AD-MSC maintained in CnT-Prime MSC-XF Medium for 4 passages.

For recommended isolation, expansion, differentiation and CFE protocols, please visit the Protocols Section of this website.

Like to try a test sample? Click here for more information.

Printable datasheet

CnT-Prime MSC-XF Medium

CnT-Prime MSC-XF Medium is a highly refined medium for high efficiency isolation and expansion of adipose-derived MSC. It uses an advanced basal medium supplemented with a range of growth factors, and is completely free of all animal-derived factors (xeno-free).

Species
Tested with human cells, may be used with other species as well.
Tissue type
MSC
Pack size
500 mL bottle, fully supplemented with growth factors. No further additions required.
Product use
Developed specifically for optimal isolation efficiency and proliferation of adMSC. Recommended seeding density immediately after isolation: 20'000 cells per sq. cm. Recommended seeding density after passaging: 2'000 cells per sq. cm. See the Protocols Page for culture protocols.
Media Type
2D-Prolif
Defined
No
ACF
Yes
Culture condition
This medium is designed for use in a 5% CO2 atmosphere. For suggested seeding and culture protocols, please visit the resources section of www.cellntec.com
Note
For routine cell cultivation, CELLnTEC does not recommend the use of antibiotics / antimycotics.
Storage / Shelf life
Store frozen below -15oC. For best before date, see label. To prepare medium for use, it is preferable to thaw overnight at 4oC. Alternatively, medium may be thawed at room temperature for approx 5-6 hours. If thawing at room temperature, ensure contents do not rise above 12oC at the end of the thawing process. Once thawed, medium has a remaining shelf-life of 6-weeks when stored at 4oC in the dark. Certain culture media components are very sensitive to light. Minimize light exposure at all times.
Quality control
Media composition is tested via osmolality, pH and the concentration of various ions. Media functionality is tested by evaluating growth and morphology or primary human adMSC over at least 2 passages. Free of bacteria, fungi and mycoplasma contamination.
Shipping condition
Medium is shipped frozen.
Intended use
For research use only. Not for use in therapy or diagnostics.
Last update
2017-09-27