CELLnTEC

Features

msc_prolif

CnT-PR-MSC medium provides class-leading adMSC proliferation rates and longevity.

The new CnT-Prime MSC medium enables high efficiency isolation and class-leading proliferation rates of adipose-derived MSC (see image at left), all without the need for plate coatings!

Cells growing in CnT-Prime MSC medium also demonstrate extended longevity, and remain strongly multipotent for at least 4 passages.

It can easily be upgraded for clinical applications by replacing the FCS with human serum. Contact us at scientist@cellntec.com for further details and pricing.

The cell population is CD45-/CD31-/CD73+/CD90+/CD105+/CD34+/-, and importantly at least 10% of the cell population remains CD34-positive at passage 4.
cd34_cnt-pr-msc
Figure: CD34 expression, CnT-PR-MSC medium, passage 4.

Cells may at any time be switched to the xeno-free #CnT-PR-MSC-XF medium, or induced to differentiate following addition of the necessary differentiation supplements. Please visit the MSC homepage for more information about other Prime MSC products.

For recommended isolation, expansion, differentiation and CFE protocols, please visit the Protocols Section of this website.

Please view the Culture Notes document for specific recommendations regarding the use of the CnT-Prime MSC-CD medium.

The CnT-PR-MSC medium is currently in the beta testing phase. Please contact scientist@cellntec.com to request your test sample.

A flyer summarising the CnT-Prime MSC expansion media is available here.

Printable datasheet

CnT-Prime MSC Medium

CnT-Prime MSC Medium is a highly refined medium for high efficiency isolation and expansion of adipose-derived MSC. It uses an advanced basal medium supplemented with a range of growth factors, plus a low level of FCS.

Species
Tested with human cells, may be used with other species as well.
Tissue type
MSC
Pack size
500 mL bottle, fully supplemented with growth factors. No further additions required.
Product use
Developed specifically for optimal isolation efficiency and proliferation of adMSC. Recommended seeding density immediately after isolation: 20'000 cells per sq. cm. Recommended seeding density after passaging: 2'000 cells per sq. cm. See the Protocols Page for culture protocols.
Media Type
2D-Prolif
Defined
No
Culture condition
This medium is designed for use in a 5% CO2 atmosphere. For suggested seeding and culture protocols, please visit the resources section of www.cellntec.com
Note
For routine cell cultivation, CELLnTEC does not recommend the use of antibiotics / antimycotics.
Storage / Shelf life
Store frozen below -15oC. For best before date, see label. To prepare medium for use, it is preferable to thaw overnight at 4oC. Alternatively, medium may be thawed at room temperature for approx 5-6 hours. If thawing at room temperature, ensure contents do not rise above 12oC at the end of the thawing process. Once thawed, medium has a remaining shelf-life of 6-weeks when stored at 4oC in the dark. Certain culture media components are very sensitive to light. Minimize light exposure at all times.
Quality control
Media composition is tested via osmolality, pH and the concentration of various ions. Media functionality is tested by evaluating growth and morphology or primary human adMSC over at least 2 passages. Free of bacteria, fungi and mycoplasma contamination.
Shipping condition
Medium is shipped frozen.
Intended use
For research use only. Not for use in therapy or diagnostics.
Last update
2016-11-21