During the air-lift culture, the volume of medium per insert plays a critcial role in how the model establishes. If the volume of medium is low, daily medium changes are reuqired, also on weekends.
At larger medium volumes, it is possible to use a convenient 3x-weekly medium-change routine (i.e. Monday, Wednesday, Friday), and still obtain an optimal structure of the epithelial model.
Standard 12-well plates typically deliver only a very small volume of medium in the range of 0.5 mL per insert (depending on the manufacturer) during air-lift culture, and thus require daily medium changes.
Deep well plates are one possible solution, but are significantly more expensive, and generally often require very large volumes of medium (3-5 mL per insert).
The #CnT-SP Spacer Plate fits on top of standard 12-well plages, and solves the medium volume problem by lifting the inserts, and thus increasing medium volume without needing a special deep-well plate.
Importantly, the #CnT-SP Spacer Plate is re-usable. Simply sterilize by soaking in alcohol prior to use.