Epithelium 2D


Primary human corneal cells (HCEP) growing in CnT-PR medium.

Proliferation Media

CnT-Prime medium is carefully formulated to deliver excellent growth and longevity of corneal epithelial cells in a fully defined, animal component free environment.

This is achieved with a combination of Progenitor Cell Targeted (PCT) factors to improve retention of cells in an undifferentiated phenotype, and co-factors to improve growth factor binding and proliferation.

Like to try a low cost test sample? Click here for a list of available sample packs.

Double your isolation efficiency from every valuable biopsy! Try the CnT-ISO-50 supplement.

For information about previous media such as CnT-20, please visit our Previous Products Page for more info.


Confluent epithelial cells can be induced to differentiate by switching to CnT-PR-D medium and adding 1.2 mM calcium.

Differentiation Media

The differentiation process of epithelial progenitor cells is a complex and highly-orchestrated series of events.

It is significantly affected by the cell’s environment.

Proliferation media contain a wide range of factors that are directly inhibitory to cell differentiation. As a result, these media actively retard the differentiation process.

The new CnT-Prime 2D Differentiation medium is specifically designed to encourage complete differentiation of epithelial progenitors, including those isolated from corneal tissue.

For differentiation if iPS and ES cells into corneal epithelium, the CnT-30 medium is recommended.

Products table

CAT# Description Species Defined Media type
CnT-PR CnT-Prime, Epithelial Culture Medium Human Yes 2D-Prolif
CnT-PR-D CnT-Prime 2D Diff, Epithelial Culture Medium Human Yes 2D-Diff
CnT-30 CnT-Prime iPS Epithelial Differentiation Medium Human and Mouse Yes 2D-Diff
CnT-ISO-50 CnT-IsoBoost Supplement Yes