CnT-PR-3D_02

3D Differentiation Media

Differentiation and stratification of epidermal keratinocytes in air-lift culture places divergent demands on the culture medium

The medium must send proliferative signals to retain the basal layer, but must also permit cells to fully differentiate and form an effective barrier.

The 3D Barrier medium is specifically designed to deliver this delicate balance. It is able to retain the basal layer, but in parallel allows more complete differentiation and a 50% improved barrier compared with regular media (more info).


Like to try a test sample? Click here for a list of available sample packs.

Looking for Full Thickness 3D models? Please click here for more information about our media for 3D skin co-culture models.

3D_Epidermis_sml

3D epidermal model, day 12, established using 3D Starter Kit. The use of the new 3D Barrier medium improves differentiation and barrier function by 50%.

3D Starter Kits

Everything you need to establish 3D epidermal models simply, reliably and cost effectively in your own lab.

Kits come with media for 2D and 3D culture, plus 50 inserts and a complete protocol.

Kits are available both with and without primary epidermal keratinocytes.


Looking for Full Thickness 3D models? Please click here for more information about our media for 3D skin co-culture models.

Products table

CAT# Description Species Defined ACF Media type
CnT-PR-3D CnT-Prime 3D Barrier Medium Human Yes Yes 3D-Diff
PR3D-HPEK-50 3D Keratinocyte Starter Kit including Primary Keratinocytes Human Yes Yes
PR3D-K-50 3D Keratinocyte Starter Kit, without Primary Keratinocytes Human Yes Yes
CnT-PR-FTAL CnT-PR-FTAL Human Yes Yes 3D-Diff
CnT-PR-FTAL5 CnT-PR-FTAL5 Human Yes No 3D-Diff
CnT-ABM10 Pen / Strep / Amphotericin B Solution, Single Aliquots Yes Yes
CnT-GAB10 Gentamycin / Amphotericin B Solution, Single Aliquots Yes Yes
CnT-DNP-10 Dispase II, Neutral Protease, 5 x 0.1 gram No No