CELLnTEC

Features

HDFs primary human dermal fibroblasts (single donor) deliver strong growth and extended longevity due to their juvenile origin, and the strong proliferative signals provided by the CnT-PR-F medium.

The HDF fibroblasts are provided cryopreserved in the fully defind CRYO-Defined culture medium, to maximise recovery after thawing.

The cultures undergo a range of QC tests in our lab before being released for sale, and are also covered by our longevity guarantee. Please see the datasheet for details.

The thawing, seeding and passaging protocols are particularly important for optimal cell growth. Please visit our Protocols Page for our recommendations.

Please visit our publications database and search by tissue type to obtain a range of articles that have used CELLnTEC primary cells and media in this specific field.

Printable datasheet

Dermal Fibroblasts, Single Donor

Normal human dermal fibroblasts, cryo preserved at P2.

This material is of human origin and should therefore be handled as hazardous. Although it has been tested negative for HIV 1, Hepatitis B and Hepatitis C, treat this material as potentially infectious, and use appropriate biocontainment, protective equipment and other precautions to prevent accidental exposure.
Species
Human, single donor, foreskin
Tissue type
Mesenchyme
Pack size
HDFs is a vial containing > 5 x 105 viable cells (1 mL)
Defined
No
ACF
No
Culture medium
CnT-PR-F
Cultivation
For cultivation instructions, please see general cultivation protocol, on our resources section.
Passaging
Recommended seeding density in CnT-PR-F after passaging: 1'000 cells / cm2. For passaging instructions, please see general cultivation protocol in the resources section of www.cellntec.com
Av. time to confluence
5 to 6 days (depending on temperature, seeding density and protocol)
Longevity
Guaranteed to provide 20 population doublings when grown in the recommended medium.
Storage / Shelf life
Immediately upon arrival transfer the cryo vial to the liquid nitrogen container, until ready to use.
Thawing
Recommended seeding density after thawing: 4'000 cells / cm2. For thawing instructions, please see general cultivation protocol in the resources section of www.cellntec.com
Freezing
Recommended freezing density: 1 x 106 cells / mL. For freezing instructions, please see general cultivation protocol in the resources section of www.cellntec.com
Quality control
Tested negative for Hepatitis B, Hepatitis C and HIV-1. Free of bacteria, fungi and mycoplasma contamination.
Shipping condition
Cells are shipped on dry ice.
Comments
For routine cell cultivation CELLnTEC recommends to work without antibiotics / antimycotics.
Intended use
For research use only. Not for use in therapy or diagnostics.
Last update
2015-07-27