CELLnTEC

Features

HPEKs primary human epidermal keratinocytes (single donor) growing in CnT-PR medium benefit from the presence of PCT factors that aid retention of proliferative progenitors, and reduce differentiation.

This extends not only the lifepsan of the cells, but importantly also the period during which the cells retain the ability to complete complex processes such as 3D differentiation.

The HPEK cells are provided cryopreserved in the fully defind CRYO-Defined culture medium, to maximise recovery after thawing.

The cultures undergo a range of QC tests in our lab before being released for sale, and are also covered by our longevity guarantee. Please see the datasheet for details.

The thawing, seeding and passaging protocols are particularly important for optimal cell growth. Please visit our Protocols Page for our recommendations.

Please visit our publications database and search by tissue type to obtain a range of articles that have used CELLnTEC primary cells and media in this specific field.

Printable datasheet

Epidermal Keratinocyte Progenitors, Single Donor

Normal human epidermal keratinocyte progenitor cells, isolated from juvenile donor material, cryo preserved at P2.

This material is of human origin and should therefore be handled as hazardous. Although it has been tested negative for HIV 1, Hepatitis B and Hepatitis C, treat this material as potentially infectious, and use appropriate biocontainment, protective equipment and other precautions to prevent accidental exposure.
Species
Human, juvenile, single donor
Tissue type
Epithelia
Pack size
Vial containing 5 x 105 viable cells (1 mL)
Defined
Yes
ACF
No
Culture medium
CnT-PR
Cultivation
For cultivation instructions, please see general cultivation protocol, on our resources section.
Passaging
Recommended seeding density after passaging: 4 x 103 cells / cm2. For passaging instructions, please see general cultivation protocol in the resources section of www.cellntec.com
Av. time to confluence
5 to 7 days (depending on temperature, seeding density and protocol)
Longevity
Guaranteed to provide 15 population doublings when grown in CnT-Prime medium.
Storage / Shelf life
Immediately upon arrival transfer the cryo vial to the liquid nitrogen container, until ready to use.
Thawing
Recommended seeding density after thawing: 4 x 103 cells / cm2. For thawing instructions, pleplease see general cultivation protocol in the resources section of www.cellntec.com
Freezing
Recommended freezing density: 1 x 106 cells / mL. For freezing instructions, please see general cultivation protocol in the resources section of www.cellntec.com
Quality control
Tested negative for Hepatitis B, Hepatitis C and HIV-1. Free of bacteria, fungi and mycoplasma contamination.
Shipping condition
Cells are shipped on dry ice.
Comments
For routine cell cultivation CELLnTEC recommends to work without antibiotics / antimycotics.
Intended use
For research use only. Not for use in therapy or diagnostics.
Last update
2015-11-12