ISOLATION PROTOCOLS

 

TISSUE TYPE	PRODUCTS USED
Primary human keratinocytes	CnT-PR , CnT-XP3 , CnT-ISO-50 , CnT-Accutase-100 , CnT-DNP-10 , CnT-GAB-10
Primary mouse keratinocytes using CnT-07 medium	CnT-07 , CnT-DNP-10 , CnT-Accutase-100 , CnT-GAB-10 , CnT-ISO-50
Primary human airway epithelial cells from a section of trachea	CnT-PR-A , CnT-DNP-10 , CnT-Accutase-100 , CnT-ISO-50
Mesenchymal stem cells from subcutaneous adipose tissue	CnT-PR-MSC , CnT-PR-MSC-XF , CnT-XP3 , CnT-GAB-10
Primary human mammary epithelial cells	CnT-PR , CnT-DNP-10 , CnT-Accutase-100 , CnT-GAB-10 , CnT-ISO-50
Primary human oral epithelial cells	CnT-PR , CnT-DNP-10 , CnT-Accutase-100 , CnT-GAB-10 , CnT-ISO-50
Human corneal epithelial cells	CnT-PR , CnT-DNP-10 , CnT-GAB-10 , CnT-Accutase-100 , CnT-ISO-50
Primary human urothelial cells from a bladder biopsy	CnT-PR , CnT-DNP-10 , CnT-GAB-10 , CnT-ISO-50
Primary human melanocytes	CnT-40 , CnT-DNP-10 , CnT-GAB-10 , CnT-Accutase-100
Primary human dermal fibroblasts	CnT-PR-F , CnT-DNP-10 , CnT-GAB-10 , CnT-Accutase-100

 
 

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PROLIFERATION & 2D/3D DIFFERENTIATION PROTOCOLS

 

APPLICATION	PRODUCTS USED
General thawing, passaging, and freezing protocol	CnT-PR , Other media , CnT-CRYO-50 , CnT-Accutase-100
Aging of keratinocytes with VitroAge	HPEK cells , CnT-PR , CnT-AG2 , CnT-Accutase-100
2D keratinocytes differentiation	HPEK cells , CnT-PR , CnT-PR-D
3D Epidermal keratinocyte cultures using Starter Kit	PR3D-HPEK-50 Starter Kit , CnT-Accutase-100
3D Epidermal keratinocyte cultures using Starter Kit without cells	PR3D-K-50 Starter Kit , CnT-Accutase-100
Full thickness in vitro skin models at the air-liquid interface	PRFT-CELLS-24 Starter Kit , CnT-Accutase-100
Adipogenic differentiation of primary human mesenchymal stem cells	CnT-PR-MSC , CnT-PR-MSC-XF , CnT-PR-MSCDIFF-AD.S
2D differentiation of large airway epithelial cells	CnT-PR-A , CnT-PR-AD
3D differentiation of large airway epithelial cells	CnT-PR-A , CnT-PR-AD
2D differentiation of Melanocytes and total melanin assay	HEM cells , CnT-40 , CnT-PR-MD , CnT-Accutase-100

 
 

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OTHER CELL CULTURE-RELATED PROTOCOLS

 

APPLICATION	PRODUCTS USED
Sample Testing Recommendations	CnT-Accutase-100 , CnT-CRYO-50
Routine histological processsing, H&E Staining and Immunhistology
OCT embedding and cryosectioning of 3D epidermal cultures
ECM induction and total collagen measurement	CnT-PR-F , CnT-PR-ECM
Transfection of epithelial keratinocytes using PEI transfection method	Epithelial culture media , CnT-PR-BM.1
Lipid Extraction from 3D epidermal models
Staining of cell colonies and inserts	CnT-ST-100

 
 

Last modified: 06-04-2023.