Actin dynamics regulate immediate PAR-2-dependent responses to acute epidermal permeability barrier abrogation
Roelandt T, Heughebaert C, Verween G, Giddelo C, Verbeken G, Pirnay JP, Devos D, Crumrine D, Roseeuw D, Elias PM, Hachem JP
Department of Dermatology, Universitair Ziekenhuis Brussel
Journal of Dermatological Science
Background: Lamellar body (LB) secretion and terminal differentiation of stratum granulosum (SG) cells are signaled by both protease activated receptor-2 (PAR-2) and caveolin-1 (cav-1). Objective: To address the early dynamics of LB secretion, we examined cytoskeletal remodeling of keratinocytes in 3 mouse models following acute barrier abrogation: hairless mice, PAR-2 knockout (-/-) and cav-1 -/-. Methods and Results: Under basal conditions, globular (G)-actin accumulates in SG cells cytosol, while filamentous (F)-actin is restricted to peri-membrane domains. Barrier abrogation induces the apical movement of F-actin and the retreat of the SG-G-actin front, paralleled by upstream cytoskeletal kinases activation. This phenomenon was both enhanced by PAR-2 agonist, and inhibited by cytochalasin-D and in PAR-2 knockout mice. We found that plasma membrane conformational changes causing LB secretion are controlled by PAR-2-dependent cytoskeletal rearrangements. We next addressed the interaction dynamics between cytoskeleton and plasma membrane following PAR-2-induced actin stress fiber formation in both cav-1 -/- and wildtype cells. Actin stress fiber formation is increased in cav-1 -/- cells prior to and following PAR-2 agonist peptide-treatment, while absence of cav-1 inhibits E-cadherinmediated cell-to-cell adhesion. Conclusion: PAR-2 drives cytoskeletal/plasma membrane dynamics that regulate early LB secretion following barrier abrogation, stress fiber formation and keratinocyte adhesion.
Product use
Isolation and cultivation for various treatments, with consequent fixation and staining for F-actin and E-cadherin, and confocal microscope visualization.
Tissue type
Tissue info
Neonatal skin from cav-1 -/- and +/+ mice
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