Primary human dermal fibroblasts growing in CnT-PR-F medium. Day 3 after seeding.

Tailored Media for Proliferation, ECM Production and Homeostasis

Traditional fibroblast media utilise 10% serum combined with very simple basal media formulations.

The new CnT-PR-F medium uses an advanced basal medium formulation and mix of defined growth factors to reduce the serum requirement down to 1%.

As a result, the new CnT-PR-F significantly increases isolation efficiency (50%) and growth rate (+40%), whilst reducing serum-associated variability.

Low cost test samples of this new medium are now available for testing.

Cells undergoing rapid proliferation are less responsive to differentiation and ECM-related triggers.

To obtain fully in-vivo like ECM production, we offer the new fully defined CnT-PR-ECM medium with a less proliferative formulation that delivers 5x higher ECM levels compared with the proliferation medium CnT-PR-F.

Low cost test samples of this new medium are now available for testing.

Cells growing in media designed for rapid proliferation or high ECM production are subject to a strong set of stimulii from the culture medium.

This can significantly reduce the responsiveness of cells to experimental stimulii.

For maximum responsiveness in your assays, we recommend the use of the CnT-Prime Fibroblast Homeostasis medium.

Traditional Fibroblast Medium

CnT-05 is a traditional 10% serum fibroblast proliferation medium.

In addition to serum, it also containsnd several additional factors.

For improved isolation efficiency, proliferation rate and ECM responsiveness, the new Prime media are recommended.

Products table

CAT# Description Species Defined Media type
CnT-PR-F CnT-Prime Fibroblast Medium Human, Mouse, and Rat No 2D-Prolif
CnT-PR-ECM CnT-Prime ECM Human Yes 2D-Diff
CnT-PR-FH CnT-Prime Fibroblast Homeostasis Medium Human Yes 2D-Prolif
CnT-05 Fibroblast Medium Human and Mouse No 2D-Prolif