Balance 2

Co-cultures require a medium that maintains the delicate balance of proliferation and functionality for both cell types

2D Keratinocyte / Fibroblast Co-culture

Standard proliferation media do not deliver well balanced growth of 2 or more cell types in co-culture.

The new CnT-PR-CC co-culture omits the fibroblast inhibitory elements found in keratinocyte media, whilst in parallel providing an environment that also encourages moderate fibroblast growth in balance with the keratinocytes in regular 2D culture.

For 3D co-culture (full thickness skin model), the CnT-PR-FTAL is recommended (see below).

Low cost test samples of CnT-PR-CC are now available for groups wishing to evaluate this product.

FT Model

Full thickness skin model with fully natural dermal ECM, prepared using CnTPR-F followed by CnT-PR-FTAL for air-lift culture.

3D Co-Culture: Full Thickness Skin Models

Traditional methods for preparation of full-thickness skin models depended in the use of collagen I gels for the establishment of the dermal compartment.

This significantly increases complexity, cost and variability.

Using our novel high-ECM culture media, it is now possible to establish full thickness skin models without the use of collagen gels.

The entire dermal matrix is secreted by the fibroblasts, creating a fully natural and complex mix of ECM proteins.

This approch is simpler, faster, and more reproducible. For more details, please visit the features page.

This model was recently featured at the TEDD 3R conference. Please click here to view the conference report.

CnT-Prime Airlift is also available in a 500 mL pack size: #CnT-PR-FTAL5

Primary keratinocytes and fibroblasts that have been tested for the establishment of FT models are also available as a separate Value Pack.

For the full written protocol describing establishing of the FT models, please visit our Protocols Page.

For more information about the reusable Spacer Plates (#CnT-SP) that increase medium volume in standard 12-well plates during air-lift culture, please click here.

Kits for Full Thickness Skin Models

Would you like the convenience of ordering a kit with everything needed to establish FT skin models in your own lab?

CELLnTEC offers two different options, one including primary keratinocytes and fibroblasts, and one without.

Products table

CAT# Description Species Defined Media type
CnT-PR-CC CnT-Prime Co-Culture, Epithelial/Stromal Medium Human Yes 2D-Prolif
CnT-PR-FTAL CnT-PR-FTAL Human Yes 3D-Diff
CnT-PR-FTAL5 CnT-PR-FTAL5 Human Yes 3D-Diff
CnT-FT-CELLS Full Thickness Primary Cell Value Pack Human Yes
PRFT-CELLS-24 3D FT Starter Kit (With Cells) Human Yes
PRFT-24 3D FT Starter Kit (No Cells) Human Yes
CnT-SP 12-well Spacer Plate Yes