Melano1

Primary human melanocytes growing in CnT-40.

Proliferation Media

CnT-40 medium is carefully formulated to deliver excellent isolation, selectivity, and rapid proliferation of primary human melanocytes.

Melanocytes grown in CnT-40 demonstrate a proliferative phenotype, typically with 2 or 3 dendrites per cell.

Moderate melanin expression is observed in the proliferative melanocytes growing in CnT-40. However when more complete differentiation and melanogenesis is required, the melanocyte differentiation medium (#CnT-PR-MD, see below) is recommended.

Low cost test samples of this medium are now available.

Total Melanin

Significantly increased melanin expression in CnT-PR-MD differentiation medium.

Differentiation and Assay Media

The Prime MD Melanocyte Differentiation medium is carefully formulated only for differentiation, and delivers 2.5x more melanin and increased dendricity of primary human melanocytes.

Melanocytes growing in CnT-PR-MD medium are less proliferative, and as a result display a much more in vivo-like phenotype.

Cells growing in a medium designed for rapid proliferation or complete differentiation are receiving a strong set of signals from the culture medium.

These strong signals tend to reduce the responsiveness of cells to the external stimulii applied during experimentation.

To improve assay responsiveness, we have developed a melanocyte homeostasis medium known as Prime MH, in which cells demonstrate higher responsiveness to assay treatments, such as tyrosinase inhibitors for example.

Products table

CAT# Description Species Defined Media type
CnT-40 Melanocyte Medium Human No
CnT-PR-MD Melanocyte Differentiation Medium Human Yes 2D-Diff
CnT-PR-MH Melanocyte Homeostasis Medium Human No 2D-Diff