Viability and Metabolism
Metabolism is a broad term that includes a wide variety of processes. It is frequently investigated in vitro, in a number of different ways.
Metabolic activity is most commonly investigated using the MTT test. This test evaluates a colour changes induced by oxidoreductase enzymes found primarily in the mitochondria of the cell.
A variety of other mitochondrial features may also be investigated to evaluate different metabolic mechanisms, including the integrity of the mitochondrial membrane, or the electron transport chain via mitochondrial membrane potential.
A more functional approach to metabolic activity can also be taken be evaluating cell proliferation. This approach can also be used to evaluate effects over longer periods, which may be important prior to certain tests, or for actives that do not have an immediate influence on cell behaviour.