Adipocyte progenitors, known as pre-adipocytes, can be cultured in standard adherent culture.
They can then be induced to differentiate over several weeks, during which time the develop the characteristic morphology and lipid droplets of mature adipocytes.
The formation of lipid droplets, lipogenesis, can be influenced by active ingredients. As mature adipocytes also secrete a variety of growth factors and other signals that influence the structure of the skin and wound healing, the differentiation of these cells is also an important process for skin function.
Differentiation and lipogenesis can be evaluated in the presence and absence of active ingredients to determine if there is a beneficial effect on adipocyte maturation.