CnT-NX-EX NEXT GENERATION EXTENDED PROLIFERATION MEDIUM
CnT-NX-EX is is a next-generation medium delivers efficient isolation and prolonged expansion of primary epithelial cells. It is crafted to overcome challenges like early senescence and limited lifespan of primary cells in defined culture conditions.
Primary human adult keratinocytes cultured at high passage 18 in CnT-NX-EX medium shows superior growth & impeccable cell morphology.
CnT-NX-EX Product Page
- Extended Growth Potential:
Achieve robust keratinocyte growth and more population doublings, redefining longevity beyond conventional media limitation.
- High Yield and Scalability:
Minimizes premature senescence, supports exponential expansion, and increases yields up to a trillion-fold for large-scale applications.
- Prolonged Experimental Timelines:
Delivers high proliferation rates, steady long-term cultivation of keratinocytes, and increases assay time windows up to 100 days.
- Excellent Morphology & Stem Cell Functionality:
Retains the morphology and functionality of primary keratinocytes at high passages, ensuring superior cell quality for sensitive 3D in vitro modeling.
- Reliable Performance:
Enhanced population doubling and longevity support reproducibility, allowing technical replicates from the same sample, and minimizing the need for sourcing additional cells or patient material.
- Innovative Formulation for Ease of Use:
Chemically defined, human or animal components-free and ROCK inhibitor-free composition that does not need feeder cells or surface coating. Streamline your workflow, reduce setup time and costs.
Comparative Analysis of Long-term Cultivation of Primary Epithelial Cells:
In our comparative analysis primary human keratinocytes were cultured in CnT-NX-EX medium, its predecessors CnT-07 and CnT-PR and three other commercially available media. Only keratinocytes grown in CnT-NX-EX achieved over 70 population doublings (at passage 19 since isolation) and their lifespan was extended beyond 100 days in culture. CnT-NX-EX outcompeted all other tested media with regards to proliferation rate, cell yield and longevity.
Prolonged Cultivation of Primary Human Juvenile and Adult Keratinocytes:
In our long-term culture assay CnT-NX-EX supported beyond 90 population doublings (at passage 21) in juvenile keratinocytes and beyond 60 population doublings (at passage 18) in adult keratinocytes. CnT-NX-EX significantly expanded lifespan of juvenile keratinocytes beyond 118 days and that of adult keratinocytes beyond 103 days in culture.
Colony Forming Efficiency of Adult Keratinocytes at Higher Passages:
Adult keratinocytes cultured in CnT-NX-EX, its predecessors CnT-07 and CnT-PR and three other commercially available media. Keratinocytes cultured in CnT-NX-EX showed excellent colony forming efficiency and outcompeted other tested media by 1.5-fold. Colonies were not observed for other tested media beyond the fourth passage. Whereas, with CnT-NX-EX colonies were observed even at higher passages 14 and 19.
In vitro 3D Epidermal Modeling:
Adult keratinocytes cultured in CnT-NX-EX, its predecessors CnT-07 and CnT-PR and three other commercially available media. Epidermal models were created by 3D air-liquid interface culture using CnT-PR-3D medium. Left panel: 3D epidermal models created with adult keratinocytes cultured in CnT-NX-EX showed better performance as compared to other tested media. Right panel: 3D epidermal models with adult keratinocytes expanded in CnT-NX-EX at passage 14 showed excellent cell morphology. No other tested media were able to create a well differentiated 3D model at higher passages.
PROTOCOLS:
Recommended optimized protocols for epithelial cells
► General cultivation || ► Human epidermal keratinocyte isolation || ► Keratinocyte isolation from other tissues
► Keratinocyte differentiation – 2D culture || ► Keratinocyte differentiation – 3D air-liquid interface culture
Learn more about our epithelial cells media suitable for iPSC derived cells, cell lines and other applications
► Media Comparison Table
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| ORDERING:
Order CnT-NX-EX and related products directly or via our distributors network.
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| TEST SAMPLES:
Procure free test materials of CnT-NX-EX and related products.
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| HIGHER CERTIFIED MEDIA (HC)
Our next-generation CnT-NX-EX medium can also be made available in a clinically ready HC version.
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| TECHNICAL SUPPORT:
Contact scientists in our R&D team for support in accelerating scientific discovery.
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