CnT-NX-EX is a next-generation medium delivers efficient isolation and prolonged expansion of primary epithelial cells. It is crafted to overcome challenges like early senescence and limited lifespan of primary cells in defined culture conditions.
Highlights of ready-to-use CnT-NX-EX:
- Extended Growth Potential:
Achieve robust keratinocyte growth and more population doublings, redefining longevity beyond conventional media limitation. - High Yield and Scalability:
Minimizes premature senescence, supports exponential expansion, and increases yields up to a trillion-fold for large-scale applications. - Prolonged Experimental Timelines:
Delivers high proliferation rates, steady long-term cultivation of keratinocytes, and increases assay time windows up to 100 days. - Excellent Morphology & Stem Cell Functionality:
Retains the morphology and functionality of primary keratinocytes at high passages, ensuring superior cell quality for sensitive 3D in vitro modeling. - Reliable Performance:
Enhanced population doubling and longevity support reproducibility, allowing technical replicates from the same sample, and minimizing the need for sourcing additional cells or patient material. - Innovative Formulation for Ease of Use:
Chemically defined, human or animal components-free and ROCK inhibitor-free composition that does not need feeder cells or surface coating. Streamline your workflow, reduce setup time and costs.
In our comparative analysis primary human keratinocytes were cultured in CnT-NX-EX medium, its predecessors CnT-07 and CnT-PR and three other commercially available media. Only keratinocytes grown in CnT-NX-EX achieved over 70 population doublings (at passage 19 since isolation) and their lifespan was extended beyond 100 days in culture. CnT-NX-EX outcompeted all other tested media with regards to proliferation rate, cell yield and longevity.
Adult keratinocytes cultured in CnT-NX-EX, its predecessors CnT-07 and CnT-PR and three other commercially available media. Epidermal models were created by 3D air-liquid interface culture using CnT-PR-3D medium. Left panel: 3D epidermal models created with adult keratinocytes cultured in CnT-NX-EX showed better performance as compared to other tested media. Right panel: 3D epidermal models with adult keratinocytes expanded in CnT-NX-EX at passage 14 showed excellent cell morphology. No other tested media were able to create a well differentiated 3D model at higher passages.
CnT-NX-EX is clinically upgradable and offers seamless transition from lab to clinical trial and ATMP-development.