CnT-17 Airway Epithelial Proliferation Medium

CnT-17

Basal medium + supplement pack

500 mL

CHF 369.00

Description

CnT-17 is a fully defined, low-calcium medium designed for the isolation and expansion of large airway epithelial cells from mouse and human tissues. It is the original formulation from which CnT-PR-A was developed and remains a reliable choice for foundational airway epithelial cell culture.
The medium is provided as a liquid culture kit, including both basal medium and supplements. For maximum isolation efficiency, it is recommended to use IsoBoost (#CnT-ISO-50), which has been shown to double cell yield during the first week of culture.

Category

Highlights

1

Isolation and expansion of airway epithelial cells

Medium for efficient isolation and expansion of large airway epithelial cells

2

Chemically defined

Chemically defined medium formulation

3

Feeder free, no coating required

Stable, feeder-free culture without plate coatings, minimizing batch-to-batch variability and preparation steps

CnT-17

CnT-PR-A

Tissue type
Lung, Airway
Airway, Lung
Cell type
Epithelial Cells
Epithelial Cells
Species
Human, may also work for other species
Human, may also work for other species
Workflow Steps
Isolation, Proliferation
Isolation, Proliferation
Serum Level
Serum-free
Serum-free
BPE Level
BPE-free
BPE-free
ACF Status
Contains animal components
Free of animal and human components
Chemically defined
Yes
Yes
Clinically upgradable
Volume
500 mL
500 mL
Component(s)
Quality Level
Research Grade
Research Grade

Scientific Literature

H Yuguri et al.

(2017)

— Oncogene

Targeting prohibitins with chemical ligands inhibits KRAS-mediated lung tumours

Yu-Zhi Lu et al.

(2020)

— Exp Cell Res

Bleomycin induced apical-basal polarity loss in alveolar epithelial cell contributes to experimental pulmonary fibrosis

Shuai-Jun Chen et al.

(2024)

— Cell Commun Signaling

DEC1 is involved in circadian rhythm disruption-exacerbated pulmonary fibrosis

Laure M.G. Petit et al.

(2024)

— Heliyon

Glypican-3 is a key tuner of the Hedgehog pathway in COPD

Adrien Maupas et al.

(2025)

— PLoS One

Roscovitine enhances the bactericidal activity of the airway surface liquid of the cystic fibrosis bronchial epithelium but does not protect against Pseudomonas aeruginosa infection

Damien Adam et al.

(2018)

— J Cystic Fibrosis

CFTR rescue with VX-809 and VX-770 favors the repair of primary airway epithelial cell cultures from patients with class II mutations in the presence of Pseudomonas aeruginosa exoproducts

Ruth A. Elderfield et al.

(2015)

— J General Virology

Ferret airway epithelial cell cultures support efficient replication of influenza B virus but not mumps virus

Fabiana C. P. Valera et al.

(2018)

— J Allergy Clin Immunol

Staphylococcus aureus impairs sinonasal epithelial repair: Effects in patients with chronic rhinosinusitis with nasal polyps and control subjects

Downloads

General thawing, passaging, and freezing protocol

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Related Products

CnT-17 has been developed for proliferation of airway epithelial cells. For 2D or 3D differentiation of these cells, it is recommended to switch to CnT-PR-AD.

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