CnT-30 iPSC Epithelial Differentiation Medium

Differentiation of human iPSC and ESC down Eye lineages in a chemically defined medium.

CnT-30

Basal medium + supplement pack

500 mL

CHF 479.00

Description

CnT-30 is a chemically defined liquid culture medium kit designed for the differentiation of human induced pluripotent stem cells (iPSCs) and embryonic stem cells (ESCs) into Eye and epidermal epithelial lineages. Its formulation supports a variety of differentiation protocols, making it a versatile tool for stem cell research.
This medium has been successfully used in multiple studies. One example is the differentiation of iPSCs into Eye epithelium followed by proteomic comparison with primary limbal cells, published in Nature Scientific Reports (Mikhailova et al., 2015). Other publications describing Eyel differentiation of hPSCs include Sorkio et al., 2018 and Hongisto et al., 2018.

Category Media

Highlights

1

Succeed in Eye iPSC/ESC differentiation

Expansion and differentiation of human iPSC and ESC down Eye lineages

2

Chemically defined

Chemically defined medium formulation

CnT-30

CnT-PR-EF

Tissue type

Eye

Eye

Cell type

Epithelial Cells, Keratinocytes

Epithelial Cells, Keratinocytes

Species

Human, may also work for other species

Human, may also work for other species

Workflow Steps

–

–

Serum Level

Serum-free

Serum-free

BPE Level

BPE-free

BPE-free

ACF Status

Contains animal components

Free of animal and human components

Chemically defined

Yes

Yes

Clinically upgradable

Please enquire

Please enquire

Volume

500 mL

500 mL

Component(s)

–

–

Quality Level

Research Grade

Research Grade

Scientific Literature

Changbin Sun et al.

(2021)

— Cell & Bioscience

Time-course single-cell RNA sequencing reveals transcriptional dynamics and heterogeneity of limbal stem cells derived from human pluripotent stem cells

Read publication >

Michael J. Edel et al.

(2025)

— Stem Cells Dev

An Optimized Method to Produce Human-Induced Pluripotent Stem Cell-Derived Limbal Stem Cells Easily Adaptable for Clinical Use

Read publication >

Maija Kauppila et al.

(2025)

— Acta Biomaterialia

Compartmentalized 3D bioprinting of the limbal niche with distinct hPSC-LSC subpopulations for corneal disease modeling

Read publication >

Meri Vattulainen et al.

(2021)

— Stem Cell Res & Ther

Corneal epithelial differentiation of human pluripotent stem cells generates ABCB5+ and ∆Np63α+ cells cells with limbal cell characteristics and high wound healing capacity

Read publication >

Anni Sorkio et al.

(2018)

— Biomaterials

Human stem cell based corneal tissue mimicking structures using laser-assisted 3D bioprinting and functional bioinks

CnT-30, CnT-PR-CC

Read publication >

Meri Vattulainen et al.

(2019)

— Stem Cell Res & Ther

Modulation of Wnt/BMP pathways during corneal differentiation of hPSC maintains ABCG2-positive LSC population that demonstrates increased regenerative potential

CnT-30, CnT-07, CnT-PR-CC

Read publication >

Maija Kauppila et al.

(2022)

— nano select

Multi-parametric surface plasmon resonance-based intake quantification of label-free light-activated nanoparticles by therapeutic limbal stem cells for corneal blindness

Read publication >

Maija Kauppila et al.

(2023)

— Adv Healthcare Mater

Toward Corneal Limbus In Vitro Model: Regulation of hPSC-LSC Phenotype by Matrix Stiffness and Topography During Cell Differentiation Process

Read publication >

Downloads

CnT-30 iPSC Epithelial Differentiation Medium Datasheet

Download

CnT-30 iPSC Epithelial Differentiation Medium SDS

Download

Flyer: CnT-ISO-50 IsoBoost Supplement and CnT-30 iPS differentiation

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Protocol: General thawing, passaging, & freezing

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