CnT-30 iPSC Epithelial Differentiation Medium

CnT-30

Basal medium + supplement pack

500 mL

CHF 479.00

Description

CnT-30 is a chemically defined liquid culture medium kit designed for the differentiation of human induced pluripotent stem cells (iPSCs) and embryonic stem cells (ESCs) into Eyel and epidermal epithelial lineages. Its formulation supports a variety of differentiation protocols, making it a versatile tool for stem cell research.
This medium has been successfully used in multiple studies. One example is the differentiation of iPSCs into Eyel epithelium followed by proteomic comparison with primary limbal cells, published in Nature Scientific Reports (Mikhailova et al., 2015). Other publications describing Eyel differentiation of hPSCs include Sorkio et al., 2018 and Hongisto et al., 2018.

Category

Highlights

1

Succeed in Eyel iPSC/ESC differentiation

Expansion and differentiation of human iPSC and ESC down Eyel lineages

2

Chemically defined

Chemically defined medium formulation

CnT-30

CnT-PR-EF

Tissue type
Eye
Eye
Cell type
Epithelial Cells, Keratinocytes
Epithelial Cells, Keratinocytes
Species
Human, may also work for other species
Human, may also work for other species
Workflow Steps
Serum Level
Serum-free
Serum-free
BPE Level
BPE-free
BPE-free
ACF Status
Contains animal components
Free of animal and human components
Chemically defined
Yes
Yes
Clinically upgradable
Volume
500 mL
500 mL
Component(s)
Quality Level
Research Grade
Research Grade

Scientific Literature

Changbin Sun et al.

(2021)

— Cell & Bioscience

Time-course single-cell RNA sequencing reveals transcriptional dynamics and heterogeneity of limbal stem cells derived from human pluripotent stem cells

Michael J. Edel et al.

(2025)

— Stem Cells Dev

An Optimized Method to Produce Human-Induced Pluripotent Stem Cell-Derived Limbal Stem Cells Easily Adaptable for Clinical Use

Maija Kauppila et al.

(2025)

— Acta Biomaterialia

Compartmentalized 3D bioprinting of the limbal niche with distinct hPSC-LSC subpopulations for corneal disease modeling

Meri Vattulainen et al.

(2021)

— Stem Cell Res & Ther

Corneal epithelial differentiation of human pluripotent stem cells generates ABCB5+ and ∆Np63α+ cells cells with limbal cell characteristics and high wound healing capacity

Anni Sorkio et al.

(2018)

— Biomaterials

Human stem cell based corneal tissue mimicking structures using laser-assisted 3D bioprinting and functional bioinks

Meri Vattulainen et al.

(2019)

— Stem Cell Res & Ther

Modulation of Wnt/BMP pathways during corneal differentiation of hPSC maintains ABCG2-positive LSC population that demonstrates increased regenerative potential

Maija Kauppila et al.

(2022)

— nano select

Multi-parametric surface plasmon resonance-based intake quantification of label-free light-activated nanoparticles by therapeutic limbal stem cells for corneal blindness

Maija Kauppila et al.

(2023)

— Adv Healthcare Mater

Toward Corneal Limbus In Vitro Model: Regulation of hPSC-LSC Phenotype by Matrix Stiffness and Topography During Cell Differentiation Process

Downloads

General thawing, passaging, and freezing protocol

Download

Related Products

Please see related product recommendations below or browse our products hub.

Contact Orders Directly