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CnT-40 Melanocyte Proliferation Medium

Selective isolation and rapid expansion of primary epidermal melanocytes.

CnT-40

Frozen medium

500 mL

CHF 284.00

Description

CnT-40 is specifically designed for the selective isolation and rapid expansion of primary epidermal melanocytes and can be upgraded for clinical applications. It contains Progenitor Cell Targeted (PCT) factors to help retain a proliferative phenotype, along with a range of melanocyte-specific factors, including endothelin-1 and endothelin-3, and 1% serum. The formulation is free of PMA and TPA and does not contain antibiotics or antimycotics.
Melanocytes cultured in CnT-40 display a primarily proliferative bipolar phenotype while retaining melanin expression.

Category

Highlights

1

Succesfully isolate and expand melanocytes

Selective isolation and rapid expansion of primary epidermal melanocytes.

2

Achieve high selectivity and rapid growth

A very selective medium featuring rapid growth & expansion.

3

Low serum medium

Low serum medium, does not contain PMA or TPA, thus enhancing reproducibility and minimizing backgound compared to other commercial formats in this class

4

Maximize standardization and convenience

Fully supplemented, one bottle ready-to-go. Maximize standardization and convenience. Minimize variability and error

5

Feeder free, no coating required

Stable, feeder-free culture without plate coatings, minimizing batch-to-batch variability and preparation steps

6

Clinically upgradable

Realize a seamless transition from lab to clinical trial and ATMP-development

 

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CnT-40

Tissue type
Skin, Eye
Cell type
Melanocytes
Species
Human, may also work for other species
Workflow Steps
Isolation, Proliferation
Serum Level
< 1 % bovine serum
BPE Level
BPE-free
ACF Status
Contains animal components
Chemically defined
No
Clinically upgradable
Yes
Volume
500 mL
Component(s)
–
Quality Level
Research Grade

Scientific Literature

Naresh Polisetti et al.

(2022)

— Cells

P-Cadherin Is Expressed by Epithelial Progenitor Cells and Melanocytes in the Human Corneal Limbus

CnT-40
Read publication >

Dominika Zielinska et al.

(2023)

— Sci Adv

Combining bioengineered human skin with bioprinted cartilage for ear reconstruction

CnT-57, CnT-40
Read publication >

Karolina Niska et al.

(2025)

— Toxicology Vitro

Cytotoxicity of ZnO nanoparticles in human melanocyte cells in the presence or absence of UV radiation: A preliminary comparative study with TiO2 nanoparticles

HEMas, CnT-40
Read publication >

J�lia Capp Zilles et al.

(2025)

— ACS Omega

Polymeric Nanocapsules Containing Kojic Acid Dipalmitate and Rosehip Oil: Development and Evaluation of Preliminary Efficacy and Safety for Skin Whitening

CnT-40
Read publication >

Bishal Tandukar et al.

(2025)

— Nat Commun

Genetic evolution of keratinocytes to cutaneous squamous cell carcinoma

CnT-40
Read publication >

St�phanie Almeida Scalvino et al.

(2018)

— Int J Cosmetic Sci

Efficacy of an agonist of α‐MSH, the palmitoyl tetrapeptide-20, in hair Pigmentation

CnT-40, HEMas
Read publication >

Naresh Polisetti et al.

(2020)

— Sci Rep

Isolation and enrichment of melanocytes from human corneal limbus using CD117 (c-Kit) as selection marker

CnT-40
Read publication >

Bonnie C. Carney et al.

(2021)

— PLoS One

Hypopigmented burn hypertrophic scar contains melanocytes that can be signaled to re-pigment by synthetic alpha-melanocyte stimulating hormone in vitro

CnT-40
Read publication >
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Downloads

CnT-40 Melanocyte Proliferation Medium Datasheet

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CnT-40 Melanocyte Proliferation Medium SDS

Download

Protocol: Melanocytes 2D differentiation & total melanin assay

Download

Protocol: General thawing, passaging, & freezing

Download

Protocol: Isolation of primary human melanocytes

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Related Products

CnT-40 has been developed for proliferation of melanocytes. For differentiation or homeostasis experiments, it is recommended to switch to the CnT-PR-MH or CnT-PR-MD media. For co-culture with keratinocytes, it is recommended to use CnT-PR-KM.

CnT-40-HC

CnT-PR-MD

For the differentiation of melanocytes, and encourages the production of higher levels of melanin and dendrites.

CnT-PR-MH

Low growth factor medium used for homeostatic maintenance of melanocytes.

CnT-PR-KM

2D co-culture of keratinocytes and melanocytes

CnT-CRYO-50

A cryopreservation medium containing all the necessary components to protect cells during freezing and thawing.

CnT-Accutase-100

Our recommended enzyme for cell detachment and passaging due to its gentle action, and the fact that no separate reagent is required to stop the reaction (simply dilute, then centrifuge).

HEMas

Normal human epidermal melanocytes, isolated from adult donor material, cryo preserved at early passage.

HEMns

Normal human epidermal melanocytes, isolated from juvenile donor material, cryo preserved at early passage.

Contact Orders Directly

orders@cellntec.com