CnT-57 Epithelial Proliferation Medium, Low BPE

CnT-57

Basal medium + supplement pack

500 mL

CHF 316.00

Description

CnT-57 is specifically designed for the isolation and expansion of epithelial cells from skin, Eye, gingiva, mammary, and bladder tissue. It is a low-calcium medium formulation based on CnT-07, supplemented with a low concentration of BPE to enhance performance.
The medium is provided as a liquid culture kit, including both basal medium and supplements.

Category Media

Highlights

1

Isolation and expansion of epithelial cells

Medium for isolation and expansion of epithelial cells from multiple tissue types

2

Low level BPE

Contains a low level of BPE, thus enhancing reproducibility and minimizing backgound compared to other commercial formats in this class

3

Feeder free, no coating required

Stable, feeder-free culture without plate coatings, minimizing batch-to-batch variability and preparation steps

CnT-57

CnT-NX-E

CnT-PR

CnT-07

Tissue type

Skin, Bladder, Oral, Other epithelial tissue, Eye

Skin, Bladder, Oral, Other epithelial tissue, Eye

Skin, Bladder, Oral, Other epithelial tissue, Eye

Skin, Bladder, Oral, Other epithelial tissue, Eye

Cell type

Epithelial Cells, Keratinocytes

Epithelial Cells, Keratinocytes

Epithelial Cells, Keratinocytes

Epithelial Cells, Keratinocytes

Species

Human, may also work for other species

Human and mouse, may also work for other species

Human and mouse, may also work for other species

Human and mouse, may also work for other species

Workflow Steps

Isolation, Proliferation

Isolation, Proliferation

Isolation, Proliferation

Isolation, Proliferation

Serum Level

Serum-free

Serum-free

Serum-free

Serum-free

BPE Level

Low level of BPE

BPE-free

BPE-free

BPE-free

ACF Status

Contains animal components

Free of animal and human components

Free of animal and human components

Contains animal components

Chemically defined

No

Yes

Yes

Yes

Clinically upgradable

–

–

–

–

Volume

500 mL

500 mL

500 mL

500 mL

Component(s)

–

–

–

–

Quality Level

Research Grade

Research Grade

Research Grade

Research Grade

	NEXT GENERATION MEDIA		FIRST GENERATION MEDIA
	CnT-NX-EX►	CnT-NX-E►	CnT-PR►	CnT-07►	CnT-57►
Media Format
Fully supplemented, ready-to-use	■	■	■
Kit with supplements				■	■
Media Characteristics
Chemically defined	■	■	■	■
Animal/Human component-free	■	■	■
No coating required	■	■	■	■	■
Applicable Cell Source
Adult primary cells	■	■	■	■	■
Juvenile primary cells	■	■	■	■	■
iPSC derived cells		■		■
Cell lines		■	■	■	■
Media Performance
Robust isolation and expansion	■	■	■	■	■
Excellent morphology & stem cell functionality	■	■	■	■	■
High growth rate	■	■
Extended longevity	■
Higher yield	■

Scientific Literature

Eunjeong Kwon et al.

(2018)

— Nat Commun

The RNA-binding protein YBX1 regulates epidermal progenitors at a posttranscriptional level

CnT-07, CnT-DNP, CnT-57

Read publication >

Yan Gao et al.

(2023)

— Cell Death Discovery

SARS-CoV-2 infection causes periodontal fibrotic pathogenesis through deregulating mitochondrial beta-oxidation

Read publication >

Florian Peters et al.

(2021)

— Matrix Biol

Syndecan-1 shedding by meprin β impairs keratinocyte adhesion and differentiation in hyperkeratosis

Read publication >

Luca Pontiggia et al.

(2022)

— Cells

Human Basal and Suprabasal Keratinocytes Are Both Able to Generate and Maintain Dermo-Epidermal Skin Substitutes in Long-Term In Vivo Experiments

Read publication >

Markus Rimann et al.

(2015)

— J Lab Automation

Standardized 3D Bioprinting of Soft Tissue Models with Human Primary Cells

Read publication >

Luca Mazzone et al.

(2016)

— Pediatric Surg

Successful grafting of tissue-engineered fetal skin

Read publication >

Ileana Bortolomai et al.

(2019)

— Stem Cells Translational Med

Gene Modification and Three-Dimensional Scaffolds as Novel Tools to Allow the Use of Postnatal Thymic Epithelial Cells for Thymus Regeneration Approaches

Read publication >

R�mi Doucet-Ladeveze et al.

(2019)

— Front Pediatr

Transcriptomic Analysis Links Eosinophilic Esophagitis and Atopic Dermatitis

Read publication >

Downloads

CnT-57 Epithelial Proliferation Medium, Low BPE Datasheet

Download

CnT-57 Epithelial Proliferation Medium, Low BPE SDS

Download

General thawing, passaging, and freezing protocol

Download

Related Products

For defined and animal-component free culture of epithelial cells, use CnT-NX-E or CnT-NX-EX media.

CnT-NX-E

Isolation and rapid expansion of primary epithelial cells from multiple tissue types, iPSC derived cells, and cell lines. Chemically defined, contains no components of animal or human origin.

CnT-NX-EX

Isolation and substantially prolonged expansion of primary epithelial cells derived from multiple tissue types. Chemically defined, contains no components of animal or human origin.

PR3D-K-50

Contains the media and inserts needed to establish 3D epidermal models with improved differentiation and barrier function in your own lab.

PRFT-24

Contains all the media necessary to establish full thickness 3D skin models from primary keratinocyte and fibroblast cultures in your own lab.

CnT-PR-H

Low growth factor medium for homeostatic maintenance of epithelial cells.

CnT-PR-D

2D differentiation of epithelial cells in a chemically defined medium.

CnT-ISO-50

A chemically defined supplement added to the culture medium after isolation, that significantly improves viability and attachment of primary epithelial cells.

HPEKp

Normal human epidermal keratinocyte progenitor cells, isolated from juvenile donor material, cryo preserved at early passage.

Contact Orders Directly