CnT-CRYO Defined, Animal Component-Free Freezing Medium

CnT-CRYO-50

Frozen

50 mL

CHF 142.00

Description

CRYO‑Defined is a chemically defined, animal‑component‑free cryopreservation medium formulated to protect cells during freezing and thawing. Supplied as a 2× concentrate, it contains 20% DMSO along with all necessary components required to maintain cell viability and integrity throughout the cryopreservation process. The medium is ready to use as provided.
Developed using only synthetic compounds, CRYO‑Defined offers a xeno‑free and fully defined alternative to traditional freezing media that rely on variable additives such as serum. In testing, CRYO‑Defined has been shown to deliver cell recovery rates equal to or better than those achieved with high‑serum cryopreservation formulations, providing a reliable and reproducible solution for maintaining cell quality through freeze–thaw cycles.

Category Reagents

Highlights

1

High efficiency cryopreservation

contains all the necessary components to protect cells during freezing and thawing

2

Fully defined and ACF

Chemically defined, contains no components of animal or human origin

3

Serum-free

Does not require serum

4

Ease of use

ready to use 2x concentrate

CnT-CRYO-50

HPEKas

HEMas

HDFas

Tissue type

Vascularization, Mesenchyme, Other epithelial tissue, Eye, Skin, Bladder, Oral, Airway, Lung

Skin

Skin

Skin

Cell type

Keratinocytes, Epithelial Cells, Fibroblasts, Melanocytes, MSCs, Endothelial Cells

Epithelial Cells, Keratinocytes

Melanocytes

Fibroblasts

Species

Human, may also work for other species

Human

Human

Human

Workflow Steps

Cryopreservation

Proliferation, Differentiation, 3D models, Co-Culture, Homeostasis, Cryopreservation

Proliferation, Differentiation, 3D models, Co-Culture, Homeostasis, Cryopreservation

Co-Culture, Homeostasis, Cryopreservation, Proliferation, Differentiation, 3D models

Serum Level

Serum-free

–

–

–

BPE Level

BPE-free

–

–

–

ACF Status

Free of animal and human components

–

–

–

Chemically defined

Yes

–

–

–

Clinically upgradable

–

–

–

–

Volume

50 mL

1 mL

1 mL

1 mL

Component(s)

–

–

–

–

Quality Level

Research Grade

Research Grade

Research Grade

Research Grade

Scientific Literature

Erin N. Burns et al.

(2018)

— Animal Genet

Generation of an equine biobank to be used for Functional Annotation of Animal Genomes project

CnT-09, CnT-CRYO-50, CnT-Accutase-100

Read publication >

David G. Belair et al.

(2017)

— PLoS One

Engineering human cell spheroids to model embryonic tissue fusion in vitro

CnT-PR, CnT-Accutase-100, CnT-PR-CC, CnT-CRYO-50, HPEKp

Read publication >

Pierre Balmer et al.

(2021)

— J Cell Biol

SUV39H2 epigenetic silencing controls fate conversion of epidermal stem and progenitor cells

CnT-09, CnT-07, CnT-DNP, CnT-CRYO-50, CnT-Accutase-100, CnT-GAB-5, HPEKp

Read publication >

Aline Chary et al.

(2022)

— Toxicology Vitro

Maximizing the relevance and reproducibility of A549 cell culture using FBS-free media

CnT-PR-A, CnT-CRYO-50

Read publication >

Downloads

CnT-CRYO Defined, Animal Component-Free Freezing Medium Datasheet

Download

CnT-CRYO Defined, Animal Component-Free Freezing Medium SDS

Download

General thawing, passaging, and freezing protocol

Download

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