CnT-Dispase II, Neutral Protease

CnT‑DNP contains Dispase II, a neutral protease that provides gentle yet effective enzymatic action for the isolation of primary epithelial cells. Dispase II cleaves fibronectin, collagen IV, and, to a lesser extent, collagen I, enabling the separation of epithelial tissue from the underlying mesenchyme. This controlled dissociation supports the recovery of high‑purity epithelial populations suitable for seeding into primary culture.
The enzyme’s mild activity helps preserve cell viability and function during isolation, making it an optimal choice for establishing primary epithelial cultures. CnT‑DNP is supplied as an approximately 100 mg powder aliquot corresponding to 96 units of activity. Because enzymatic activity may vary between lots, the exact powder mass can differ.
Each 100 mg aliquot is sufficient to prepare 40 mL of a 1× working solution, yielding a final concentration of 2.4 U/mL. This volume supports approximately two to four tissue isolations, depending on the size of the specimens processed.