CnT-Gentamycin / Amphotericin B Solution, 500 x, Ready-to-Use Single Aliquots

CnT-GAB-5

Frozen

5 x 1 mL of 500x concentrate

CHF 91.00

Description

CnT‑GAB‑5 is a convenient, ready‑to‑use antibiotic/antimycotic supplement designed to provide broad and stable protection against microbial contamination during primary cell isolation and passage 1. Supplied as a sterile 500× concentrate in single‑use aliquots, it contains gentamycin sodium salt and amphotericin B (Fungizone). When diluted to working concentration, the medium contains 10 µg/mL gentamycin and 0.25 µg/mL amphotericin B.
Traditional antibiotics such as penicillin/streptomycin are not ideal for routine cell culture due to their instability. Gentamycin offers several advantages, including a broader effective antimicrobial spectrum, higher thermo‑stability at 37 °C, longer shelf life, and greater resistance to acidic or alkaline conditions, retaining more than 90% activity for up to two weeks.
CnT‑GAB‑5 provides dependable contamination protection during the critical early stages of cell establishment. However, antibiotics should only be used during isolation and passage 1. Their use in later passages, and particularly during challenging processes such as differentiation, is not recommended because they may interfere with normal cell behavior.

Category

Highlights

1

Broad efficiency

Broader effective spectrum

2

Stability

Higher thermo-stability at 37°C and acid / alkali resistance than penicillin/streptomycin

3

Ease of use

ready to use 500x concentrate

CnT-GAB-5

Tissue type
Skin, Bladder, Oral, Airway, Lung, Vascularization, Mesenchyme, Other epithelial tissue, Eye
Cell type
Epithelial Cells, Fibroblasts, Melanocytes, MSCs, Endothelial Cells, Keratinocytes
Species
n/a
Workflow Steps
Isolation
Serum Level
Serum-free
BPE Level
BPE-free
ACF Status
Free of animal and human components
Chemically defined
Yes
Clinically upgradable
Volume
5 x 1 mL
Component(s)
Quality Level
Research Grade

Scientific Literature

Pierre Balmer et al.

(2021)

— J Cell Biol

SUV39H2 epigenetic silencing controls fate conversion of epidermal stem and progenitor cells

Alexis Aguiar et al.

(2021)

— Viruses

Human Cytomegalovirus Replication and Infection-Induced Syncytia Formation in Labial, Foreskin, and Fetal Lung Fibroblasts

Cinthia Silva-Vilches et al.

(2022)

— J Investig Dermatol

Topical Application of Adenosine A2-Type Receptor Agonists Prevents Contact Hypersensitivity Reactions in Mice by Affecting Skin Dendritic Cells

Lisa Marie Fr�hlich et al.

(2024)

— Int J Cancer

PARP1 expression predicts PARP inhibitor sensitivity and correlates with metastatic potential and overall survival in melanoma

ASM Sakhawat Hossain et al.

(2024)

— Matrix Biol

Fibrillin-1 G234D mutation in the hybrid1 domain causes tight skin associated with dysregulated elastogenesis and increased collagen cross-linking in mice

Katharina Bitschar et al.

(2019)

— Nat Commun

Lugdunin amplifies innate immune responses in the skin in synergy with host- and microbiota-derived factors

Yu Katata et al.

(2020)

— Cell Death & Disease

Costello syndrome model mice with a Hras G12S mutation are susceptible to develop house dust mite-induced atopic dermatitis

Downloads

General thawing, passaging, and freezing protocol

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Isolation of primary human dermal fibroblasts

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Isolation of primary human melanocytes

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Isolation of primary human urothelial cells from a bladder biopsy

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Isolation of human corneal epithelial cells

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Isolation of primary human oral epithelial cells

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Isolation of primary human mammary epithelial cells

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Isolation of mesenchymal stem cells from subcutaneous adipose tissue

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Isolation of primary mouse keratinocytes using CnT-07 medium

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Isolation of primary human keratinocytes

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