CnT-IsoBoost, Isolation Boosting Supplement

CnT-ISO-50

Frozen

50 μL of 1000x concentrate

CHF 124.00

Description

CnT‑ISO‑50 is a chemically defined supplement designed to significantly improve the viability and attachment of primary epithelial cells immediately after isolation. Supplied as a sterile 1000× ready‑to‑use concentrate, it is added to the culture medium during the first three days following isolation, when primary cells experience the greatest stress.
Isolation places substantial strain on primary cells, as physical detachment and loss of cell–cell cohesion can lead to reduced viability and weakened attachment. By supporting cells during this vulnerable period, CnT‑ISO‑50 increases overall isolation efficiency.
The IsoBoost supplement enhances early cell survival and attachment rates, helping primary human epithelial cells recover more effectively from isolation‑induced stress and establishing a more reliable starting culture.

Category

Highlights

1

Isolation booster

Significantly increases isolation efficiency

2

Fully Defined

Chemically defined, contains no components of animal or human origin

CnT-ISO-50

CnT-PR

CnT-07

Tissue type
Skin, Bladder, Oral, Airway, Lung, Other epithelial tissue, Eye
Skin, Bladder, Oral, Other epithelial tissue, Eye
Skin, Bladder, Oral, Other epithelial tissue, Eye
Cell type
Epithelial Cells, Keratinocytes
Epithelial Cells, Keratinocytes
Epithelial Cells, Keratinocytes
Species
Human, may also work for other species
Human and mouse, may also work for other species
Human and mouse, may also work for other species
Workflow Steps
Isolation
Isolation, Proliferation
Isolation, Proliferation
Serum Level
Serum-free
Serum-free
Serum-free
BPE Level
BPE-free
BPE-free
BPE-free
ACF Status
Free of animal and human components
Free of animal and human components
Contains animal components
Chemically defined
Yes
Yes
Yes
Clinically upgradable
Volume
50 μL
500 mL
500 mL
Component(s)
Quality Level
Research Grade
Research Grade
Research Grade

Scientific Literature

Huaping Zheng et al.

(2022)

— Cell Death Disease

SerpinB7 deficiency contributes to development of psoriasis via calcium-mediated keratinocyte differentiation dysfunction

Mariana Badenes et al.

(2023)

— Life Sci Alliance

The ADAM17 sheddase complex regulator iTAP/Frmd8 modulates inflammation and tumor growth

Jinrong Zeng et al.

(2022)

— Exp Dermatol

Protective roles of tRNA-derived small RNA tRF-Ile-AAT-019 in pathological progression of psoriasis

Tanja Grossmann et al.

(2023)

— PLoS One

Introducing a new type of alternative laryngeal mucosa model

Jinghao Chen et al.

(2024)

— PLOS Comput Biol

PIEZO1 regulates leader cell formation and cellular coordination during collective keratinocyte migration

Alexis Aguiar et al.

(2021)

— Viruses

Human Cytomegalovirus Replication and Infection-Induced Syncytia Formation in Labial, Foreskin, and Fetal Lung Fibroblasts

Sophie Domingues et al.

(2022)

— Cells

Clinical Grade Human Pluripotent Stem Cell-Derived Engineered Skin Substitutes Promote Keratinocytes Wound Closure In Vitro

Jesse R Holt et al.

(2021)

— eLife

Spatiotemporal dynamics of PIEZO1 localization controls keratinocyte migration during wound healing

Downloads

General thawing, passaging, and freezing protocol

Download

Isolation of primary human urothelial cells from a bladder biopsy

Download

Isolation of human corneal epithelial cells

Download

Isolation of primary human oral epithelial cells

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Isolation of primary human mammary epithelial cells

Download

Isolation of primary human airway epithelial cells from a section of trachea

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Isolation of primary mouse keratinocytes using CnT-07 medium

Download

Isolation of primary human keratinocytes

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Related Products

To maximize longevity and yield after isolation of primary epithelial cells, it is recommended to use CnT-NX-EX extended epithelial proliferation medium.

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