CnT-IsoBoost, Isolation Boosting Supplement, Higher Certified

CnT-ISO-50-HC

Frozen

50 μL

CHF 219.00

Description

CnT‑ISO‑50‑HC is a chemically defined, clinically ready supplement designed to significantly improve the viability and attachment of primary epithelial cells immediately after isolation. Primary cell isolation places considerable stress on cells, as physical detachment and the loss of cell–cell cohesion lead to reduced viability and weakened attachment. This supplement addresses these challenges by providing targeted support during the most vulnerable early phase of culture.
Supplied as a sterile, 1000× ready‑to‑use concentrate, CnT‑ISO‑50‑HC is added to the culture medium for the first three days following isolation. During this period, it strengthens cell survival and enhances attachment efficiency, leading to more reliable establishment of primary epithelial cultures.
The Higher Certified version of IsoBoost, CnT‑ISO‑50‑HC, delivers these benefits within a formulation suitable for regulated workflows. By protecting cells from isolation‑induced stress, it enables significantly improved isolation efficiency and early culture performance of primary human epithelial cells.

Category

Highlights

1

Achieve high efficiency isolation and expansion

Significantly increases isolation efficiency

2

Fully defined

Chemically defined, contains no components of animal or human origin, supports 3R principles

3

Extended support

Extended QC, documentation, support, and process control for clinical researchers

CnT-ISO-50-HC

CnT-PR-HC

CnT-07-HC

Tissue type
Oral, Airway, Lung, Cornea, Other Mucosal tissue, Skin, Bladder
Skin, Bladder, Oral, Cornea, Other Mucosal tissue
Skin, Bladder, Oral, Cornea, Other Mucosal tissue
Cell type
Epithelial Cells, Keratinocytes
Epithelial Cells, Keratinocytes
Epithelial Cells, Keratinocytes
Species
Human and mouse, may also work for other species
Human and mouse, may also work for other species
Human and mouse, may also work for other species
Workflow Steps
Isolation
Isolation, Proliferation
Isolation, Proliferation
Serum Level
serum-free
serum-free
serum-free
BPE Level
BPE-free
BPE-free
BPE-free
ACF Status
free of animal and human components
free of animal and human components
contains animal components
Chemically defined
Yes
Yes
Yes
Clinically upgradable
Volume
Component(s)
Quality Level
Higher Certified
Higher Certified
Higher Certified

Scientific Literature

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Downloads

General thawing, passaging, and freezing protocol

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Related Products

CnT-ISO-50-HC is the “higher certified” version of CnT-ISO-50. For testing and R&D purposes, it is usually suitable to use CnT-ISO-50.

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