CnT-NX-E Epithelial Proliferation Medium

CnT-NX-E

Frozen medium

500 mL

CHF 311.00

Description

CnT-NX-E is the next-generation successor to CELLnTEC’s market-leading precision media, CnT-07 and CnT-PR, designed for the isolation and expansion of epithelial cells from skin, Eye, gingiva, mammary, and bladder tissue. It offers markedly improved expansion rates, robustness, and versatility across primary cells, iPSC-derived cells, and cell lines.
This medium supports efficient isolation and expansion of primary epithelial cells from various tissue types, including keratinocytes from skin, Eyel epithelial cells, and oral epithelial cells. CnT-NX-E combines advanced proliferation rates with broad applicability for diverse research and development needs.
CnT-NX-E is chemically defined, contains no components of animal or human origin, and requires no feeder cells or plate coating. Its design ensures maximum reproducibility and excellent inter-experimental and lot-to-lot consistency. The medium is fully supplemented and provided in a single, ready-to-use bottle.
Primary epithelial cells grown in CnT-NX-E exhibit excellent morphology and maintain functionality over several passages. For differentiation experiments, switching to CnT-PR-D or CnT-PR-3D differentiation media is recommended.
CnT-NX-E is ideally suited for in vitro studies on skin and inflammatory diseases, irritation assays, cosmetic or drug evaluations, and 3D-modeling applications. It enables a seamless transition from laboratory research to clinical trials and ATMP development.

Category Media

Highlights

Achieve exceptional isolation and expansion

Achieve rapid, robust isolation and expansion of a broad range epithelial cell types including primary keratinocytes

Unmatched versatility

One solution for cultivating primary cells, iPSC-derived cells, and established cell lines

Fully defined, ROCKi-free

ROCK inhibitor-free, chemically defined, contains no components of animal or human origin, supports 3R principles

Feeder free, no coating required

Stable, feeder-free culture without plate coatings, minimizing batch-to-batch variability and preparation steps

Clinically upgradable

Realize a seamless transition from lab to clinical trial and ATMP-development

Maximize standardization and convenience

Fully supplemented, one bottle ready-to-go. Maximize standardization and convenience. Minimize variability and error

Retain morphology and stemness

Delivers excellent morphology and stem cell functionality

CnT-NX-E

CnT-PR

CnT-07

CnT-57

Tissue type

Bladder, Oral, Other epithelial tissue, Eye, Skin

Skin, Bladder, Oral, Other epithelial tissue, Eye

Cell type

Epithelial Cells, Keratinocytes

Species

Human and mouse, may also work for other species

Human, may also work for other species

Workflow Steps

Isolation, Proliferation

Serum Level

Serum-free

BPE Level

BPE-free

Low level of BPE

ACF Status

Free of animal and human components

Contains animal components

Chemically defined

Yes

Clinically upgradable

–

Volume

500 mL

Component(s)

–

Quality Level

Research Grade

CnT-NX-E_ProductPage_1140x400px — [Fig. 2]. Comparative Analysis of Long-term Cultivation of Adult Keratinocytes: The graph shows adult Keratinocytes cultured in CnT-NX-E medium exhibiting accelerated proliferation rates and achieving a higher number of population doublings in a shorter time frame compared to those grown in our predecessor media, CnT-07 and CnT-PR. CnT-NX-E effectively mitigates the issue of low initial cell counts, allowing for up to a sixfold increase in cell numbers within three passages. CnT-NX-E-HC will deliver similar results like the CnT-NX-E medium.

Primary human adult keratinocytes cultured at passage 4 in the new CnT-NX-E

CnT-NX-E is a next-generation medium designed for the efficient isolation and expansion of primary epithelial cells from various epithelial tissue types, including keratinocytes from skin, corneal epithelial cells, and oral epithelial cells. It succeeds CELLnTEC’s market leading products CnT-07 and CnT-PR.

CnT-NX-E features advanced proliferation rates and versatility for a wide range of applications for primary cells, iPSC-derived cells, and cell lines. CnT-NX-E medium is chemically defined, and contains no components of animal or human origin. No feeder cells or plate coating are required. The design ensures highest reproducibility and excellent inter-experimental and lot-to-lot consistency. This medium is fully supplemented and comes in a single bottle, ready-to-go.

The medium is ideally suited to support in vitro studies on skin and inflammatory diseases, irritation assays, and cosmetic or drug evaluations, and other 3D-modelling applications. It enables a seamless transition from lab to clinical trial and ATMP-development.

CnT-NX-E supports high cell proliferation rates over several passages. Primary epithelial cells grown in CnT-NX-E exhibit excellent morphology and maintain functionality. For differentiation experiments, it is recommended to switch to the CnT-PR-D or CnT-PR-3D differentiation media.

The medium undergoes a range of QC tests before being released for sale. Please see the datasheet for details. It can also be procured in an upgraded Higher Certified version for clinical applications.

Like to try a test sample? Click here for more information. Thawing, seeding, and passaging protocols are particularly important for optimal cell growth. Please visit our Protocols Page for our recommendations.

	NEXT GENERATION MEDIA		FIRST GENERATION MEDIA
	CnT-NX-EX►	CnT-NX-E►	CnT-PR►	CnT-07►	CnT-57►
Media Format
Fully supplemented, ready-to-use	■	■	■
Kit with supplements				■	■
Media Characteristics
Chemically defined	■	■	■	■
Animal/Human component-free	■	■	■
No coating required	■	■	■	■	■
Applicable Cell Source
Adult primary cells	■	■	■	■	■
Juvenile primary cells	■	■	■	■	■
iPSC derived cells		■		■
Cell lines		■	■	■	■
Media Performance
Robust isolation and expansion	■	■	■	■	■
Excellent morphology & stem cell functionality	■	■	■	■	■
High growth rate	■	■
Extended longevity	■
Higher yield	■

Scientific Literature

Francisco J. Roa et al.

(2025)

— Cancer Res Commun

Repurposing amiodarone for bladder cancer treatment

HBLAK, CnT-NX-E

Read publication >

C�line David et al.

(2025)

— Cell Death Dis

Small-molecule inhibitor of C-terminal HSP90 dimerization modulates autophagy and functions synergistically with mTOR inhibition to kill cisplatin-resistant cancer cells

CnT-NX-E, CnT-Accutase-100, HBLAK

Read publication >

Downloads

CnT-NX-E Epithelial Proliferation Medium Datasheet

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CnT-NX-E Epithelial Proliferation Medium SDS

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General thawing, passaging, and freezing protocol

Download

Related Products

Epithelial cells growing in CnT-NX-E display a highly proliferative phenotype. For differentiation or homeostasis experiments, it is recommended to switch to the CnT-PR-D, CnT-PR-H, or CnT-PR-3D media. For extended expansion of primary epithelial cells, use CnT-NX-EX instead.