CnT-NX-E-HC Epithelial Proliferation Medium, Higher Certified

CnT-NX-E-HC

Frozen medium

500 mL

CHF 468.00

Description

CnT-NX-E-HC is the next-generation successor to CnT-07-HC and CnT-PR-HC, CELLnTEC’s market-leading Higher Certified precision media for the isolation and expansion of epithelial cells from skin, cornea, gingiva, mammary, and bladder tissue. This clinically ready formulation offers markedly improved expansion rates, robustness, and versatility across primary epithelial cells, iPSC-derived cells, and cell lines. It is ideally suited for use during product development, protocol validation, and characterization of cell-based products.
CnT-NX-E-HC is a chemically defined, low-calcium medium designed for optimal and rapid isolation, expansion, and longevity of primary epithelial cells. It is completely free of animal or human-derived components and does not contain phenol red or antibiotics/antimycotics. The formulation includes purified growth factors, an optimized basal medium to enhance cell proliferation, and PCT factors that maximize retention of proliferative progenitor cells, increase longevity, and minimize unwanted differentiation. This medium is provided fully supplemented and frozen, ready for use in clinical workflows.

Category Media

Highlights

Next-gen successor

Next generation precision medium in a clinically ready formulation

Achieve exceptional isolation and expansion

Achieve robust isolation and expansion of a broad range epithelial cell types including primary keratinocytes

Unmatched versatility

One solution for cultivating primary cells, iPSC-derived cells, and established cell lines

Retain morphology and stemness

Delivers excellent morphology and stem cell functionality. Seamless transition from lab to clinical trial and ATMP-development.

Fully defined, ROCKi-free

ROCK inhibitor-free, chemically defined, contains no components of animal or human origin

Feeder free, no coating required

No feeder cells or plate coating required. Fully supplemented, one bottle ready-to-go.

Extended support

Extended QC, documentation, support, and process control for clinical researchers

CnT-NX-E-HC

CnT-PR-HC

CnT-07-HC

Tissue type

Skin, Bladder, Oral, Cornea, Other Mucosal tissue

Cell type

Epithelial Cells, Keratinocytes

Species

Human and mouse, may also work for other species

Workflow Steps

Isolation, Proliferation

Serum Level

serum-free

BPE Level

BPE-free

ACF Status

free of animal and human components

contains animal components

Chemically defined

Yes

Clinically upgradable

–

Volume

–

Component(s)

–

Quality Level

Higher Certified

CnT-NX-E_atP4-150x150 — [Fig. 1]. Primary human adult keratinocytes cultured at passage 4 in CnT-NX-E. CnT-NX-E and CnT-NX-E-HC have same media formulation and deliver similar results

CnT-NX-E_ProductPage_1140x400px — [Fig. 2]. Comparative Analysis of Long-term Cultivation of Adult Keratinocytes: The graph shows adult Keratinocytes cultured in CnT-NX-E medium exhibiting accelerated proliferation rates and achieving a higher number of population doublings in a shorter time frame compared to those grown in our predecessor media, CnT-07 and CnT-PR. CnT-NX-E effectively mitigates the issue of low initial cell counts, allowing for up to a sixfold increase in cell numbers within three passages. CnT-NX-E-HC will deliver similar results like the CnT-NX-E medium.

Scientific Literature

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Downloads

CnT-NX-E-HC Epithelial Proliferation Medium, Higher Certified Datasheet

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CnT-NX-E-HC Epithelial Proliferation Medium, Higher Certified SDS

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General thawing, passaging, and freezing protocol

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Related Products

CnT-NX-E-HC is the “higher certified” version of CnT-NX-E. For testing and R&D purposes, it is usually suitable to use CnT-NX-E. For extended culture of primary cells, consider testing CnT-NX-EX, which can also be made available in a “higher certified” version.