CnT-NX-EX Epithelial Proliferation Medium

CnT-NX-EX

Frozen medium

500 mL

CHF 321.00

Description

CnT-NX-EX is a next-generation culture medium designed for the efficient isolation and prolonged expansion of primary epithelial cells from various tissue types, including keratinocytes from skin, Eyel epithelial cells, oral epithelial cells, and other epithelial subtypes. Compared to other commercially available formats, CnT-NX-EX significantly enhances derivation and expansion efficiency.
CnT-NX-EX achieves up to 90 population doublings in human neonatal keratinocytes and 60 doublings in adult keratinocytes, enabling a potential trillion-fold increase in cell numbers over the culture duration. This efficiency allows researchers to perform repeats and technical replicates from the same sample, reducing the need for additional cell sourcing or patient material. Extended cellular longevity also increases experimental or assay time windows to 100 days or more.
CnT-NX-EX supports high proliferation rates while mitigating premature cellular senescence. Cells grown in this medium exhibit excellent morphology, maintain functionality, and retain the ability to differentiate into all epithelial layers even at high passage numbers. For differentiation experiments, switching to CnT-PR-D or CnT-PR-3D differentiation media is recommended.
The formulation is ROCK inhibitor-free, chemically defined, and contains no components of animal or human origin. No feeder cells or plate coating are required. Its design ensures maximum reproducibility and excellent inter-experimental and lot-to-lot consistency. The medium is fully supplemented and provided in a single, ready-to-use bottle.
CnT-NX-EX is ideally suited for in vitro studies on skin and inflammatory diseases, irritation assays, cosmetic or drug evaluations, and 3D-modeling applications. It enables a seamless transition from laboratory research to clinical trials and ATMP development.

Category Media

Highlights

Obtain higher yields

More population doublings of primary keratinocytes and other epithelial cells compared to other commercial media

Maximize your experimental window

Benefit from greatly extended longevity and experimental time window

Retain long-term stemness

Delivers excellent morphology and stem cell functionality up to high passages

Fully defined, ROCKi-free

ROCK inhibitor-free, chemically defined, contains no components of animal or human origin, supports 3R principles

Feeder free, no coating required

Stable, feeder-free culture without plate coatings, minimizing batch-to-batch variability and preparation steps

Clinically upgradable

Realize a seamless transition from lab to clinical trial and ATMP-development

Maximize standardization and convenience [OR: Standardization and convenience]

Fully supplemented, one bottle ready-to-go. Maximize standardization and convenience. Minimize variability and error

CnT-NX-EX

CnT-PR

Tissue type

Airway, Other epithelial tissue, Eye, Skin, Bladder, Oral

Other epithelial tissue, Eye, Skin, Bladder, Oral

Cell type

MSCs, Keratinocytes, Epithelial Cells, Fibroblasts, Melanocytes

Epithelial Cells, Keratinocytes

Species

Human and mouse, may also work for other species

Workflow Steps

Isolation, Proliferation

Serum Level

Serum-free

BPE Level

BPE-free

ACF Status

Free of animal and human components

Chemically defined

Yes

Clinically upgradable

–

Volume

–

500 mL

Component(s)

–

Quality Level

Research Grade

NX-EX_Keartino_Culture — [Fig. 1]. Primary human adult keratinocytes cultured at higher passages 6 and 18 in the new CnT-NX-EX shows superior growth and impeccable cell morphology.

NX-EX_TwoDonor_1140x400px_300ppi — [ Fig. 2]. Long-term Cultivation of Human Neonatal and Adult Keratinocytes: CnT-NX-EX supports up to 90 population doublings (PDs) in neonatal keratinocytes which is significantly higher than 40 PDs obtained with predecessor CnT-PR. Likewise, CnT-NX-EX supports up to 70 PDs in adult keratinocytes as compared to 25 PDs obtained with CnT-PR.

NX-EX_Comp-Graph_1140x400px — [ Fig 3]. Comparative Analysis of Long-term Cultivation of Adult Human Keratinocytes: Primary cells were cultured in CnT-NX-EX medium, and its predecessors CnT-07 and CnT-PR and three other commercially available medium. Only keratinocytes grown in CnT-NX-EX showed enhanced proliferation rates, achieved a higher number of population doublings of up to 70 PDs, and extended the lifespan of keratinocytes for up to 110 days (and beyond) in culture. CnT-NX-EX outcompeted all other tested media.

	NEXT GENERATION MEDIA		FIRST GENERATION MEDIA
	CnT-NX-EX►	CnT-NX-E►	CnT-PR►	CnT-07►	CnT-57►
Media Format
Fully supplemented, ready-to-use	■	■	■
Kit with supplements				■	■
Media Characteristics
Chemically defined	■	■	■	■
Animal/Human component-free	■	■	■
No coating required	■	■	■	■	■
Applicable Cell Source
Adult primary cells	■	■	■	■	■
Juvenile primary cells	■	■	■	■	■
iPSC derived cells		■		■
Cell lines		■	■	■	■
Media Performance
Robust isolation and expansion	■	■	■	■	■
Excellent morphology & stem cell functionality	■	■	■	■	■
High growth rate	■	■
Extended longevity	■
Higher yield	■