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CnT-Prime Epithelial 3D Airlift Medium

CnT-PR-3D

Frozen medium

500 mL

CHF 354.00

Description

CnT-PR-3D (CnT-Prime Epithelial 3D Airlift Medium) is a chemically defined formulation designed for optimal differentiation of primary human epidermal keratinocytes in 3D airlift cultures. It is completely free of animal or human-derived components and does not contain phenol red or antibiotics/antimycotics.
The medium contains a specifically tailored mix of growth factors and lipids to create an environment conducive to full differentiation and the formation of a strong epidermal barrier. This results in maximal differentiation and increased barrier function in 3D epidermal keratinocyte models.
CnT-PR-3D is used to establish 3D epidermal models from primary human keratinocytes previously expanded in CnT proliferation media. According to the recommended protocol, this can be achieved within 12 days of airlift culture. For convenience, the 3D Airlift Medium is also available as part of 3D Starter Kits, which include all components necessary for the establishment of 3D epidermal models.

Category

Highlights

1

Achieve perfection in 3D airlift culture

Superior differentiation and increased barrier function in 3D epidermal keratinocyte models

2

Maximize standardization and convenience

Fully supplemented, one bottle ready-to-go. Maximize standardization and convenience. Minimize variability and error
free sample

CnT-PR-3D

CnT-NX-E

CnT-PR

Tissue type
Skin, Bladder
Skin, Bladder, Oral, Other epithelial tissue, Eye
Other epithelial tissue, Eye, Skin, Bladder, Oral
Cell type
Epithelial Cells, Keratinocytes
Epithelial Cells, Keratinocytes
Epithelial Cells, Keratinocytes
Species
Human, may also work for other species
Human and mouse, may also work for other species
Human and mouse, may also work for other species
Workflow Steps
3D models
Isolation, Proliferation
Isolation, Proliferation
Serum Level
Serum-free
Serum-free
Serum-free
BPE Level
BPE-free
BPE-free
BPE-free
ACF Status
Free of animal and human components
Free of animal and human components
Free of animal and human components
Chemically defined
Yes
Yes
Yes
Clinically upgradable
–
–
–
Volume
500 mL
500 mL
500 mL
Component(s)
–
–
–
Quality Level
Research Grade
Research Grade
Research Grade

Scientific Literature

Harry Horsley et al.

(2018)

— Sci Rep

A urine-dependent human urothelial organoid offers a potential alternative to rodent models of infection

CnT-PR, CnT-PR-3D, HBLAK
Read publication >

Ruth Jinfen Chai et al.

(2023)

— Int J Bioprinting

Developing a bioink for single-step deposition and maturation of human epidermis

CnT-PR, CnT-PR-F, CnT-PR-3D, HDFs
Read publication >

Gijs Rikken et al.

(2023)

— Microbiome

Novel methodologies for host-microbe interactions and microbiome-targeted therapeutics in 3D organotypic skin models

CnT-PR, CnT-PR-3D
Read publication >

Lisa M. Sevilla et al.

(2024)

— Cell Death Disease

Glucocorticoid receptor controls atopic dermatitis inflammation via functional interactions with P63 and autocrine signaling in epidermal keratinocytes

HPEKp, CnT-PR, CnT-PR-3D
Read publication >

Shinobu Nakanishi et al.

(2025)

— STAR Protoc

Protocol for odorant-binding protein 2A gene knockdown by dual siRNA transfection in a three-dimensional human epidermal equivalent model

CnT-PR, CnT-PR-3D
Read publication >

Jos P.H. Smits et al.

(2020)

— J Investig Dermatol

Targeting the Cutaneous Microbiota in Atopic Dermatitis by Coal Tar via AHR-Dependent Induction of Antimicrobial Peptides

CnT-PR, CnT-PR-3D
Read publication >

Yuki Tanabe et al.

(2019)

— FEBS J

Studies on differentiation-dependent expression and activity of distinct transglutaminases by specific substrate peptides using three-dimensional reconstituted epidermis

CnT-PR, CnT-PR-3D
Read publication >

Patricia Renz et al.

(2019)

— J Cell Mol Med

Arginine‐ but not alanine‐rich carboxy‐termini trigger nuclear translocation of mutant keratin 10 in ichthyosis with confetti

CnT-PR, CnT-PR-3D, CnT-PR-D
Read publication >
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Downloads

CnT-Prime Epithelial 3D Airlift Medium Datasheet

Download

CnT-Prime Epithelial 3D Airlift Medium SDS

Download

General thawing, passaging, and freezing protocol

Download

Related Products

It is strongly recommended to use epithelial cells that are growing in CELLnTEC epithelial proliferation media such as CnT-NX-E or CnT-NX-EX, to avoid a switch in base medium composition during model formation.

CnT-NX-E

Isolation and rapid expansion of primary epithelial cells from multiple tissue types, iPSC derived cells, and cell lines. Chemically defined, contains no components of animal or human origin.

PR3D-HPEK-50

Contains everything needed to establish 3D epidermal models with improved differentiation and barrier function in your own lab.

PR3D-K-50

Contains the media and inserts needed to establish 3D epidermal models with improved differentiation and barrier function in your own lab.

CnT-PR-CC

Co-culture of epithelial and stromal cells in 2D culture.

CnT-PR-D

2D differentiation of epithelial cells in a chemically defined medium.

PRFT-24

Contains all the media necessary to establish full thickness 3D skin models from primary keratinocyte and fibroblast cultures in your own lab.

HPEKp

Normal human epidermal keratinocyte progenitor cells, isolated from juvenile donor material, cryo preserved at early passage.

HPEKs

Normal human epidermal keratinocyte progenitor cells, isolated from juvenile donor material, cryo preserved at at early passage.

HPEKas

Normal human epidermal keratinocyte progenitor cells, isolated from adult donor material, cryo preserved at early passage.

HBLAK

Spontaneously immortalised human bladder epithelial cells, cryo preserved at approx P10.

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orders@cellntec.com