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CnT-Prime Airway Epithelial Proliferation Medium

CnT-PR-A

Frozen medium

500 mL

CHF 302.00

Description

CnT-PR-A is a fully defined, chemically formulated medium designed for the isolation, expansion, and longevity of primary large airway epithelial cells. It is completely free of animal or human-derived components and does not contain phenol red or antibiotics/antimycotics. This medium is an evolution and refinement of our previous airway formulation, CnT-17.
CnT-PR-A uses an optimized basal medium enriched with trace elements, protective antioxidants, and vitamins. It is supplemented with Progenitor Cell Targeted (PCT) growth factors for efficient isolation and extended longevity, as well as co-factors that improve growth factor binding to membrane-bound receptors. These components work together to maximize retention of proliferative progenitor cells, increase cellular lifespan, and minimize unwanted differentiation.

Category

Highlights

1

Isolation and expansion of airway epithelial cells

Market leading precision medium for efficient isolation and expansion of large airway epithelial cells

2

Fully defined

Chemically defined, contains no components of animal or human origin, supports 3R principles

3

Maximize standardization and convenience

Fully supplemented, one bottle ready-to-go. Maximize standardization and convenience. Minimize variability and error

4

Feeder free, no coating required

Stable, feeder-free culture without plate coatings, minimizing batch-to-batch variability and preparation steps

5

Clinically upgradable

Realize a seamless transition from lab to clinical trial and ATMP-development
free sample

CnT-PR-A

CnT-17

Tissue type
Lung, Airway
Airway, Lung
Cell type
Epithelial Cells
Epithelial Cells
Species
Human, may also work for other species
Human, may also work for other species
Workflow Steps
Proliferation, Isolation
Isolation, Proliferation
Serum Level
Serum-free
Serum-free
BPE Level
BPE-free
BPE-free
ACF Status
Free of animal and human components
Contains animal components
Chemically defined
Yes
Yes
Clinically upgradable
Please enquire
Please enquire
Volume
500 mL
500 mL
Component(s)
–
–
Quality Level
Research Grade
Research Grade
Airway3D_Day20

Scientific Literature

Yung-Yu Yang et al.

(2020)

— Front Cell Developmental Biol

Consecutive Hypoxia Decreases Expression of NOTCH3, HEY1, CC10, and FOXJ1 via NKX2-1 Downregulation and Intermittent Hypoxia-Reoxygenation Increases Expression of BMP4, NOTCH1, MKI67, OCT4, and MUC5AC via HIF1A Upregulation in Human Bronchial Epithelial Cells

CnT-PR-A, CnT-PR-AD
Read publication >

Aline Chary et al.

(2022)

— Toxicology Vitro

Maximizing the relevance and reproducibility of A549 cell culture using FBS-free media

CnT-PR-A, CnT-CRYO-50
Read publication >

Haibin Deng et al.

(2022)

— Cell Mol Life Sci volume

Targeting lactate dehydrogenase B-dependent mitochondrial metabolism affects tumor initiating cells and inhibits tumorigenesis of non-small cell lung cancer by inducing mtDNA damage

CnT-PR-A
Read publication >

Liang Zhou et al.

(2023)

— Int J Mol Sci

Extracellular Heat Shock Protein 70 Increases the Glucocorticoid Receptor and Dual-Specificity Phosphatase 1 via Toll-like Receptor 4 and Attenuates Inflammation in Airway Epithelial Cells

CnT-PR-A
Read publication >

Michael Roth et al.

(2021)

— Pharmaceuticals

Up-regulated Vitamin D Receptor by PelargoniumSidoides Extract EPs® 7630 Contributes to Rhinovirus Defence in Bronchial Epithelial Cells

CnT-PR-A
Read publication >

Qingzhu Sun et al.

(2019)

— Asthma Basic Sci

Bronchial thermoplasty decreases airway remodelling by blocking epitheliumderived heat shock protein-60 secretion and protein arginine methyltransferase-1 in fibroblasts

CnT-PR-A, CnT-PR-F
Read publication >

Michael Roth et al.

(2019)

— PLoS One

Pelargonium sidoides radix extract EPs 7630 reduces rhinovirus infection through modulation of viral binding proteins on human bronchial epithelial cells

CnT-PR-A
Read publication >

Shun-Qing Liang et al.

(2015)

— Cell Death Disease

Blocking the epithelial-to-mesenchymal transition pathway abrogates resistance to anti-folate chemotherapy in lung cancer

CnT-PR-A
Read publication >
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Downloads

CnT-Prime Airway Epithelial Proliferation Medium Datasheet

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CnT-Prime Airway Epithelial Proliferation Medium SDS

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Protocol: 3D differentiation of large airway epithelial cells

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Protocol: 2D differentiation of large airway epithelial cells

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Protocol: General thawing, passaging, & freezing

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Protocol: Isolation of primary human airway epithelial cells from a section of trachea

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Related Products

CnT-PR-A has been developed for proliferation of airway epithelial cells. For 2D or 3D differentiation of these cells, it is recommended to switch to CnT-PR-AD.

CnT-17

Efficient isolation and expansion of large airway epithelial cells.

CnT-PR-AD

Differentiation of large airway epithelial cells in 2D and 3D culture.

CnT-ISO-50

A chemically defined supplement added to the culture medium after isolation, that significantly improves viability and attachment of primary epithelial cells.

CnT-CRYO-50

A cryopreservation medium containing all the necessary components to protect cells during freezing and thawing.

CnT-Accutase-100

Our recommended enzyme for cell detachment and passaging due to its gentle action, and the fact that no separate reagent is required to stop the reaction (simply dilute, then centrifuge).

Contact Orders Directly

orders@cellntec.com