CnT-Prime Basal Medium

CnT-PR-BM.1

Frozen medium

500 mL

CHF 138.00

Description

CnT-Prime Basal Medium, is a fully defined formulation used across the entire CnT-Prime range of media. It contains a low calcium concentration of 0.07 mM, specifically chosen to support optimal long-term proliferation of epithelial progenitor cells. This medium provides a complete set of amino acids, minerals, vitamins, and organic compounds, ensuring a balanced environment for cell maintenance and growth.

CnT-Prime Basal Medium is completely free of animal or human-derived components and does not contain growth factors, phenol red, or antibiotics/antimycotics. Growth factors must be added to achieve cell proliferation, making this basal medium ideal for applications where cells need to be starved of stimulation for a defined period prior to experimentation. It serves as the foundation for the preparation of several specialized media, including CnT-PR, CnT-PR-D, CnT-PR-3D, CnT-PR-CA, CnT-PR-A, and CnT-PR-AD.

Designed for use in a 5% CO₂ atmosphere, CnT-Prime Basal Medium combines precision and flexibility, supporting both routine culture and specialized experimental conditions.

Category Media

Highlights

CnT-PR base medium

Basal medium formulation used in the CnT-Prime (CnT-PR-…) range of cell culture media

Fully defined

Chemically defined, contains no components of animal or human origin, supports 3R principles

Feeder free, no coating required

Stable, feeder-free culture without plate coatings, minimizing batch-to-batch variability and preparation steps

CnT-PR-BM.1

CnT-PR-CSF

CnT-PR-CA

Tissue type

Mesenchyme, Other epithelial tissue, Eye, Skin, Bladder, Oral, Airway, Lung, Vascularization

Skin, Bladder, Oral, Other epithelial tissue, Eye

Cell type

Epithelial Cells, Fibroblasts, Melanocytes, MSCs, Endothelial Cells, Keratinocytes

Epithelial Cells, Keratinocytes

Species

Human, may also work for other species

Human and mouse, may also work for other species

Workflow Steps

–

Isolation, Proliferation

Serum Level

Serum-free

BPE Level

BPE-free

ACF Status

Free of animal and human components

Chemically defined

Yes

Clinically upgradable

–

Volume

500 mL

Component(s)

–

Quality Level

Research Grade

Scientific Literature

Brian E. Sansbury et al.

(2021)

— Am J Pathol

PCTR1 enhances repair and bacterial clearance in skin wounds.

HPEKp, CnT-PR, CnT-PR-BM.1

Read publication >

Fengzhen Liu et al.

(2021)

— Cell Rep

miR-24 controls the regenerative competence of hair follicle progenitors by targeting Plk3

CnT-PR, CnT-PR-BM.1

Read publication >

Ivana Gabriela Reidel et al.

(2024)

— Vaccine

Liposomal co-encapsulation of a novel gemini lipopeptide and a CpG-ODN induces a strong Th1 response with the co-activation of a Th2/Th17 profile and high antibody levels

CnT-07, CnT-PR-BM.1

Read publication >

David G. Belair et al.

(2018)

— Toxicological Sci

A Three-Dimensional Organoid Culture Model to Assess the Influence of Chemicals on Morphogenetic Fusion

CnT-PR, CnT-PR-CC, CnT-PR-BM.1, HPEKp

Read publication >

Downloads

CnT-Prime Basal Medium Datasheet

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CnT-Prime Basal Medium SDS

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General thawing, passaging, and freezing protocol

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Related Products

For homeostasis and maintenance of cells, it is recommended to use the respective homeostasis media, such as CnT-PR-H for epithelial cells, CnT-PR-MH for melanocytes, and CnT-PR-FH for fibroblasts.