CnT-Prime Epithelial Proliferation Medium, Calcium-Free

CnT-PR-CA

Frozen medium

500 mL

CHF 1.00

Description

CnT-Prime Calcium-Free is a fully defined, animal-component-free medium designed for specialized applications that require extremely low calcium levels. This formulation contains only residual amounts of calcium, and calcium must be supplemented to enable proper cell attachment and proliferation. For routine culture, the standard CnT-Prime medium with a low calcium level of 0.07 mM is recommended.
CnT-Prime Calcium-Free includes a complete range of amino acids, minerals, vitamins, and organic compounds, combined with growth factors that support strong cell proliferation. It also contains co-factors that improve growth factor binding to cell surface receptors and PCT factors that maximize retention of proliferative progenitor cells, increase longevity, and minimize unwanted differentiation. The formulation does not contain phenol red or antibiotics/antimycotics and is a modified version of the standard CnT-Prime medium. It is intended for use only in special circumstances where an unusually low calcium level is required.

Category

Highlights

1

Low calcium epithelial cell culture

Medium formulation containing only residual calcium for specialized low-calcium epithelial cell culture applications

2

Design calcium-dependent experiments

Offers all benefits of CnT-PR and allows the flexibility to freely adjust calcium concentration

3

Fully defined

Chemically defined, contains no components of animal or human origin, supports 3R principles

4

Maximize standardization and convenience [OR: Standardization and convenience]

Fully supplemented, one bottle ready-to-go. Maximize standardization and convenience. Minimize variability and error

5

Feeder free, no coating required

Stable, feeder-free culture without plate coatings, minimizing batch-to-batch variability and preparation steps

CnT-PR-CA

Tissue type
Skin, Bladder, Oral, Other epithelial tissue, Eye
Cell type
Epithelial Cells, Keratinocytes
Species
Human and mouse, may also work for other species
Workflow Steps
Proliferation, Isolation
Serum Level
Serum-free
BPE Level
BPE-free
ACF Status
Free of animal and human components
Chemically defined
Yes
Clinically upgradable
Volume
500 mL
Component(s)
Quality Level
Research Grade

Scientific Literature

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Downloads

General thawing, passaging, and freezing protocol

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Related Products

This medium contains only residual amount of calcium. For culture of epithelial cells, either add suitable amount of calcium or use our ready-to-use epithelial proliferation media (e.g. CnT-PR, CnT-NX-E, or CnT-NX-EX).

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