CnT-Prime Epithelial/Stromal Co-Culture Medium

CnT-PR-CC is a fully defined medium specifically formulated to enable balanced co-culture of primary human keratinocytes and fibroblasts in a controlled environment. This medium supports the simultaneous growth of both epithelial and stromal cells without compromising their respective phenotypes.
In practice, keratinocytes and fibroblasts are first expanded in their respective media. When seeded at a 50:50 ratio into standard 2D culture, both cell types proliferate effectively. At confluency, the cultures display the characteristic keratinocyte progenitor colonies with a small, tight cobblestone morphology, surrounded by fibroblasts.
CnT-PR-CC provides an ideal platform for studies requiring epithelial-stromal interactions in vitro, such as wound healing, tissue regeneration, and skin model development.