CnT-Prime Epithelial Proliferation Medium, Corticosteroid-Free

CnT-PR-CSF

Frozen medium

500 mL

CHF 312.00

Description

CnT-PR-CSF is a fully defined medium specifically designed for experiments or applications that require a corticosteroid-free environment. It is a modified version of the CnT-PR medium, which is widely used for the isolation and expansion of epithelial cells from skin, Eye, gingiva, mammary, and bladder tissue. While CnT-PR-CSF is formulated without corticosteroids, supplementation may be required depending on the application.
This medium retains all the benefits of CnT-PR, including being completely free of animal or human-derived components. It contains a full range of amino acids, minerals, vitamins, and organic compounds, along with growth factors that promote strong cell proliferation and co-factors that improve growth factor binding to cell surface receptors. Additionally, it includes PCT factors to maximize retention of proliferative progenitor cells, increase longevity, and minimize unwanted differentiation. The formulation does not contain phenol red or antibiotics/antimycotics.

Category

Highlights

1

Corticosteroid-free epithelial cell culture

Contains no corticosteroid

2

Design corticosteroid-free experiments

Offers all benefits of CnT-PR in a corticosteroid-free environment

3

Fully defined

Chemically defined, contains no components of animal or human origin, supports 3R principles

4

Maximize standardization and convenience [OR: Standardization and convenience]

Fully supplemented, one bottle ready-to-go. Maximize standardization and convenience. Minimize variability and error

5

Feeder free, no coating required

Stable, feeder-free culture without plate coatings, minimizing batch-to-batch variability and preparation steps

CnT-PR-CSF

Tissue type
Oral, Other epithelial tissue, Eye, Skin, Bladder
Cell type
Epithelial Cells, Keratinocytes
Species
Human and mouse, may also work for other species
Workflow Steps
Isolation, Proliferation
Serum Level
Serum-free
BPE Level
BPE-free
ACF Status
Free of animal and human components
Chemically defined
Yes
Clinically upgradable
Volume
500 mL
Component(s)
Quality Level
Research Grade

Scientific Literature

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Downloads

General thawing, passaging, and freezing protocol

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