CnT-Prime Epithelial 2D Differentiation Medium

CnT-PR-D is a chemically defined, animal-component-free formulation designed for the differentiation of epithelial cells from skin, Eye, gingiva, mammary, and bladder tissues in 2D culture. It creates an environment conducive to full differentiation while maintaining consistency and reproducibility.
The medium contains a specifically tailored mix of growth factors, where proliferative components such as PCT growth factors that retard differentiation are omitted or reduced, and pro-differentiation components are added. It uses an optimized basal medium enriched with trace elements, protective antioxidants, and vitamins. CnT-PR-D does not contain phenol red or antibiotics/antimycotics.
This formulation is an evolution and refinement of previous differentiation media, including CnT-02, CnT-30, and CnT-32. It is a low-calcium medium, as calcium plays a key role in the differentiation of confluent epithelial cells. It is recommended to add calcium at the desired time point—please refer to our differentiation protocol for detailed guidance.