CnT-Prime Fibroblast ECM Medium

CnT-PR-ECM

Frozen medium

500 mL

CHF 260.00

Description

CnT-PR-ECM is a fully defined medium specifically designed to trigger high extracellular matrix (ECM) production by fibroblasts isolated from a range of tissues. The formulation includes a tailored mix of growth factors combined with Vitamin C, which promotes collagen synthesis and ECM deposition. Its growth factor composition shifts the focus away from strong proliferation, enabling fibroblasts to produce in vivo-like quantities of ECM.

Category

Highlights

1

High ECM production from fibroblasts

Specifically designed to enable secretion of ECM proteins in fibroblasts

2

Maximizes collagen secretion

Redirect fibroblast activity from proliferation toward ECM production, to achieve ~5× increased collagen secretion

3

Fully defined

Chemically defined, contains no components of animal or human origin, supports 3R principles

4

Clinically upgradable

Realize a seamless transition from lab to clinical trial and ATMP-development

5

Feeder free, no coating required

Stable, feeder-free culture without plate coatings, minimizing batch-to-batch variability and preparation steps

6

Maximize standardization and convenience [OR: Standardization and convenience]

Fully supplemented, one bottle ready-to-go. Maximize standardization and convenience. Minimize variability and error

CnT-PR-ECM

CnT-PR-F

Tissue type
Skin, Mesenchyme
Skin, Mesenchyme
Cell type
Fibroblasts
Fibroblasts
Species
Human, may also work for other species
Human, may also work for other species
Workflow Steps
Differentiation
Isolation, Proliferation
Serum Level
Serum-free
1 % bovine serum
BPE Level
BPE-free
BPE-free
ACF Status
Free of animal and human components
Contains animal components
Chemically defined
Yes
No
Clinically upgradable
Volume
500 mL
500 mL
Component(s)
Quality Level
Research Grade
Research Grade

Scientific Literature

Nikola Kolundzic et al.

(2021)

— J Invest Dermatol Innovations

Epidermal Basement Membrane Substitutes for Bioengineering of Human Epidermal Equivalents

Marla Dubau et al.

(2024)

— J Tissue Eng

Advancing skin model development: A focus on a self-assembled, induced pluripotent stem cell-derived, xeno-free approach

ASM Sakhawat Hossain et al.

(2024)

— Matrix Biol

Fibrillin-1 G234D mutation in the hybrid1 domain causes tight skin associated with dysregulated elastogenesis and increased collagen cross-linking in mice

Marla Dubau et al.

(2025)

— J Tissue Eng

Development of an iPSC-derived immunocompetent skin model for identification of skin sensitizing substances

Julien Coutier et al.

(2022)

— J Investig Dermatol

Human-Induced Pluripotent Stem Cell-Derived Keratinocytes, a Useful Model to Identify and Explore the Pathological Phenotype of Epidermolysis Bullosa Simplex

Sophie Domingues et al.

(2022)

— Cells

Clinical Grade Human Pluripotent Stem Cell-Derived Engineered Skin Substitutes Promote Keratinocytes Wound Closure In Vitro

Downloads

ECM induction and total collagen measurement

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General thawing, passaging, and freezing protocol

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Related Products

CnT-PR-ECM has been optimized for ECM secretion of fibroblasts. For proliferation of fibroblasts, it is recommended to use CnT-PR-F.

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