CnT-Prime Fibroblast ECM Medium

Encouraging high secretion of extra cellular matrix proteins from stromal cells.

CnT-PR-ECM

Frozen medium

500 mL

CHF 260.00

Description

CnT-PR-ECM is a fully defined medium specifically designed to trigger high extracellular matrix (ECM) production by fibroblasts isolated from a range of tissues. The formulation includes a tailored mix of growth factors combined with Vitamin C, which promotes collagen synthesis and ECM deposition. Its growth factor composition shifts the focus away from strong proliferation, enabling fibroblasts to produce in vivo-like quantities of ECM.

Category Media

Highlights

1

High ECM production from fibroblasts

Specifically designed to enable secretion of ECM proteins in fibroblasts

2

Maximizes collagen secretion

Redirect fibroblast activity from proliferation toward ECM production, to achieve ~5× increased collagen secretion

3

Fully defined

Chemically defined, contains no components of animal or human origin, supports 3R principles

4

Clinically upgradable

Realize a seamless transition from lab to clinical trial and ATMP-development

5

Feeder free, no coating required

Stable, feeder-free culture without plate coatings, minimizing batch-to-batch variability and preparation steps

6

Maximize standardization and convenience

Fully supplemented, one bottle ready-to-go. Maximize standardization and convenience. Minimize variability and error

CnT-PR-ECM

CnT-PR-F

Tissue type

Skin, Mesenchyme

Skin, Mesenchyme

Cell type

Fibroblasts

Fibroblasts

Species

Human, may also work for other species

Human, may also work for other species

Workflow Steps

Differentiation

Isolation, Proliferation

Serum Level

Serum-free

1 % bovine serum

BPE Level

BPE-free

BPE-free

ACF Status

Free of animal and human components

Contains animal components

Chemically defined

Yes

No

Clinically upgradable

Please enquire

Yes

Volume

500 mL

500 mL

Component(s)

–

–

Quality Level

Research Grade

Research Grade

Scientific Literature

Nikola Kolundzic et al.

(2021)

— J Invest Dermatol Innovations

Epidermal Basement Membrane Substitutes for Bioengineering of Human Epidermal Equivalents

Read publication >

Marla Dubau et al.

(2024)

— J Tissue Eng

Advancing skin model development: A focus on a self-assembled, induced pluripotent stem cell-derived, xeno-free approach

CnT-PR, CnT-PR-ECM, CnT-PR-FTAL5

Read publication >

ASM Sakhawat Hossain et al.

(2024)

— Matrix Biol

Fibrillin-1 G234D mutation in the hybrid1 domain causes tight skin associated with dysregulated elastogenesis and increased collagen cross-linking in mice

CnT-PR, CnT-DNP, CnT-GAB-5, CnT-PR-ECM

Read publication >

Marla Dubau et al.

(2025)

— J Tissue Eng

Development of an iPSC-derived immunocompetent skin model for identification of skin sensitizing substances

CnT-PR, CnT-PR-ECM

Read publication >

Julien Coutier et al.

(2022)

— J Investig Dermatol

Human-Induced Pluripotent Stem Cell-Derived Keratinocytes, a Useful Model to Identify and Explore the Pathological Phenotype of Epidermolysis Bullosa Simplex

CnT-07, CnT-PR-ECM, CnT-07-HC, CnT-PR-FTAL5

Read publication >

Sophie Domingues et al.

(2022)

— Cells

Clinical Grade Human Pluripotent Stem Cell-Derived Engineered Skin Substitutes Promote Keratinocytes Wound Closure In Vitro

CnT-07, CnT-07-HC, CnT-PR-F, CnT-PR-FTAL5, CnT-PR-ECM, CnT-ISO-50, CnT-PR-3D, HPEKs

Read publication >

Downloads

CnT-Prime Fibroblast ECM Medium Datasheet

Download

CnT-Prime Fibroblast ECM Medium SDS

Download

Protocol: General thawing, passaging, & freezing

Download

Related Products

CnT-PR-ECM has been optimized for ECM secretion of fibroblasts. For proliferation of fibroblasts, it is recommended to use CnT-PR-F.

CnT-PR-F

High efficiency isolation and expansion of fibroblasts, without plate coating.

CnT-PR-FH

Low growth factor medium used for homeostatic maintenance of stromal cells.

HDFp

Normal human dermal fibroblasts, cryo preserved at early passage.

CnT-PR-FTAL5

Air-lift establishment of full thickness skin models featuring both fibroblasts and keratinocytes.

PRFT-24

Contains all the media necessary to establish full thickness 3D skin models from primary keratinocyte and fibroblast cultures in your own lab.

CnT-PR-CC

Co-culture of epithelial and stromal cells in 2D culture.

Contact Orders Directly