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CnT-Prime Epithelial Proliferation Medium, EGF/FGF-Free

CnT-PR-EF

Frozen medium

500 mL

CHF 299.00

Description

CnT-PR-EF is a chemically defined, animal component-free medium designed for the expansion and differentiation of human induced pluripotent stem cells (iPSCs) and embryonic stem cells (ESCs) into Eyel epithelial lineages. The formulation contains low levels of growth factors and is completely free of EGF and FGF, ensuring conditions that promote the desired differentiation pathway. It does not contain antibiotics or antimycotics.
CnT-PR-EF provides a controlled environment for guiding stem cells toward Eyel epithelial fate. For detailed instructions on how to supplement this medium to induce differentiation into Eyel epithelium, please refer to Nature Protocols (Hayashi et al., 2017).

Category

Highlights

1

Succeed in Eyel iPSC/ESC differentiation

High efficiency expansion and differentiation of human iPSC and ESC down Eyel lineages

2

Fully defined

Chemically defined, contains no components of animal or human origin, supports 3R principles

3

Maximize standardization and convenience

Fully supplemented, one bottle ready-to-go after addition of serum. Maximize convenience, minimize variability and error

4

Clinically upgradable

Realize a seamless transition from lab to clinical trial and ATMP-development
free sample

CnT-PR-EF

CnT-30

Tissue type
Eye
Eye
Cell type
Epithelial Cells, Keratinocytes
Epithelial Cells, Keratinocytes
Species
Human, may also work for other species
Human, may also work for other species
Workflow Steps
–
–
Serum Level
Serum-free
Serum-free
BPE Level
BPE-free
BPE-free
ACF Status
Free of animal and human components
Contains animal components
Chemically defined
Yes
Yes
Clinically upgradable
–
–
Volume
500 mL
500 mL
Component(s)
–
–
Quality Level
Research Grade
Research Grade

Scientific Literature

Ryuhei Hayashi et al.

(2017)

— Nat Protoc

Coordinated generation of multiple ocular-like cell lineages and fabrication of functional corneal epithelial cell sheets from human iPS cells

CnT-PR-EF
Read publication >

Ryuhei Hayashi et al.

(2022)

— Nature

Generation of 3D lacrimal gland organoids from human pluripotent stem cells

CnT-PR-EF
Read publication >

Shun Shibata et al.

(2018)

— Cell Rep

Selective Laminin-Directed Differentiation of Human Induced Pluripotent Stem Cells into Distinct Ocular Lineages

CnT-PR-EF
Read publication >

Shun Shibata et al.

(2019)

— Regenerative Ther

The secretome of adipose-derived mesenchymal stem cells attenuates epithelial-mesenchymal transition in human corneal epithelium

CnT-PR-EF
Read publication >

Yuki Kobayashi et al.

(2020)

— Stem Cell Res

Ocular Surface Ectoderm Instigated by WNT inhibition and BMP4

CnT-PR-EF
Read publication >

Kimihito Nomi et al.

(2021)

— Cell Rep

Generation of functional conjunctival epithelium, including goblet cells, from human iPSCs

CnT-PR-EF
Read publication >

Shinya Watanabe et al.

(2021)

— iScience

Human iPS cells engender corneal epithelial stem cells with holoclone-forming capabilities

CnT-PR-EF
Read publication >

Masahiro Kitao et al.

(2023)

— iScience

Identification of BST2 as a conjunctival epithelial stem/progenitor cell marker

CnT-PR-EF
Read publication >
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Downloads

CnT-Prime Epithelial Proliferation Medium, EGF/FGF-Free Datasheet

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CnT-Prime Epithelial Proliferation Medium, EGF/FGF-Free SDS

Download

General thawing, passaging, and freezing protocol

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