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CnT-Prime Endothelial Proliferation Medium w/o Serum, Higher Certified

CnT-PR-EN-HC

Frozen medium

500 mL

CHF 526.00

Description

CnT‑PR‑EN‑HC is a serum‑free, clinically ready medium designed for class‑leading proliferation of primary human endothelial cells over extended periods. It supports high‑efficiency isolation and expansion and is well suited for product development, protocol validation, and characterization of cell‑based products.
The formulation is highly refined and based on an advanced basal medium supplemented with Progenitor Cell Targeted (PCT) growth factors, which extend cellular longevity, as well as co‑factors that enhance growth factor binding to membrane‑bound receptors. It does not contain phenol red or antibiotics/antimycotics.
For optimal performance, users are advised to supplement the medium with 2% serum appropriate for their clinical application.

Category

Highlights

1

Succesfully isolate and expand endothlial cells

Achieve extended longevity of endothelial cells in culture

2

Xeno-free formulation

Xeno-free medium, contains no components of animal origin

3

Serum-free format

Serum-free formulation enables the end user to supplement with serum validated to deliver the desired cell behavior and known to meet the necessary regulatory requirements

4

Feeder free, no coating required

Stable, feeder-free culture without plate coatings, minimizing batch-to-batch variability and preparation steps

5

Extended support

Extended QC, documentation, support, and process control for clinical researchers
free sample

CnT-PR-EN-HC

Tissue type
Vascularization
Cell type
Endothelial Cells
Species
Human, may also work for other species
Workflow Steps
Isolation, Proliferation
Serum Level
Supplementation by user
BPE Level
BPE-free
ACF Status
free of animal and human components
Chemically defined
Yes
Clinically upgradable
–
Volume
–
Component(s)
–
Quality Level
Higher Certified
HUVEC_PR-EN_450x600px-150x150
[Fig. 1]. Primary HUVEC cells growing in the CnT-PR-EN medium at Passage 3
VE_Cadherin_450px-150x150
[Fig. 2]. Primary HUVEC cells demonstrate correct juntional localization of VE-Cadherin under both static and flow (1.4 Pa) conditions. Analysis courtesy of Georgios Stefanopoulos, Laboratory of Thermodynamics in Emerging Technologies, ETH Zurich.
TubeFormation_CnT-Endo_EGM-2_400px-150x150
[Fig. 3]. Tube formation (microvascular-on-a-chip) and in vitro modeling of blood clotting by primary HUVEC cells grown in CELLnTEC’s endothelial medium (upper). Red: live cell staining. Green: Fluorochrom-labelled fibrinogen. Control: cells in EGM-2 medium (lower). Image courtesy of the Schroeder Group, Experimental Haemostasis, University of Bern.

Scientific Literature

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Downloads

CnT-Prime Endothelial Proliferation Medium w/o Serum, Higher Certified Datasheet

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CnT-Prime Endothelial Proliferation Medium w/o Serum, Higher Certified SDS

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General thawing, passaging, and freezing protocol

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Related Products

For testing and R&D purposes, consider using the ready-to-use CnT-PR-EN instead.

CnT-PR-EN

High-efficiency isolation and expansion of primary human endothelial cells in an FBS-free, Xeno-Free, clinically upgradable formulation.

CnT-NX-E-HC

CnT-PR-F-HC

CnT-40-HC

CnT-CRYO-50

A cryopreservation medium containing all the necessary components to protect cells during freezing and thawing.

Contact Orders Directly

orders@cellntec.com