CnT-Prime Fibroblast Proliferation Medium w/o Serum, Higher Certified

CnT-PR-F-HC

Frozen medium

500 mL

CHF 404.00

Description

CnT‑PR‑F‑HC is a serum‑free, clinically ready medium designed for the high‑efficiency isolation and expansion of human and animal fibroblasts without requiring any plate coating. It is well suited for product development, protocol validation, and characterization of cell‑based products. Although the formulation does not contain serum, the use of 1% serum is recommended to achieve improved isolation efficiency and proliferation, offering superior performance when compared with traditional 10% serum media.
This medium is a highly refined formulation based on an advanced basal medium supplemented with a range of growth factors. The inclusion of defined growth factors and co‑factors allows the serum level to be reduced to 1%, while maintaining or exceeding the proliferation rates typically observed in high‑serum formulations. Enhanced growth factor binding to cell surface receptors supports more rapid expansion and greater consistency than conventional 10% serum media.
By reducing dependence on serum, CnT‑PR‑F‑HC provides improved reproducibility and experimental stability.

Category

Highlights

1

Achieve high efficiency isolation and expansion

High efficiency isolation combined with rapid growth and expansion of fibroblasts

2

Serum-free format

Serum-free formulation enables the end user to supplement with serum validated to deliver the desired cell behavior and known to meet the necessary regulatory requirements

3

Low 1% serum formulation

Improved isolation and proliferation of fibroblasts, and lower background and variability compared to conventional 10% serum media

4

Feeder free, no coating required

Stable, feeder-free culture without plate coatings, minimizing batch-to-batch variability and preparation steps

5

Extended support

Extended QC, documentation, support, and process control for clinical researchers

CnT-PR-F-HC

Tissue type
Skin
Cell type
Fibroblasts
Species
Human, may also work for other species
Workflow Steps
Isolation, Proliferation
Serum Level
Supplementation by user
BPE Level
BPE-free
ACF Status
free of animal and human components
Chemically defined
Yes
Clinically upgradable
Volume
Component(s)
Quality Level
Higher Certified

Scientific Literature

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Downloads

General thawing, passaging, and freezing protocol

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Related Products

For testing and R&D purposes, consider using the ready-to-use and fully supplemented CnT-PR-F instead.

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