CnT-Prime Full Thickness 3D Airlift Medium

CnT-PR-FTAL5 is a chemically defined medium specifically designed for use during the air-lift culture phase in the establishment of full-thickness (FT) skin models. These models are 3D co-cultures of primary fibroblasts and keratinocytes, and CnT-PR-FTAL5 enables their reliable construction without the need for collagen gels. This is achieved through the high levels of extracellular matrix (ECM) secreted by fibroblasts in this medium, closely mimicking in vivo conditions.
Protocol Overview for FT Model Establishment:
1) Seed fibroblasts into inserts using CnT-PR-F medium and grow in submerged culture for 14-21 days.
2) Seed keratinocytes on top of the dermal fibroblast layer and switch to CnT-PR-FTAL medium.
3) Grow the models for 3 days in submerged culture, then raise to the air-liquid interface for an additional 12 days.
CnT-PR-FTAL5 is also available as part of full-thickness skin model kits, which include all necessary components for streamlined model construction.