CnT-Prime Epithelial Homeostasis Medium

CnT-PR-H is a chemically defined medium designed for the maintenance of primary human keratinocytes in a homeostatic state for up to one week. It provides only minimal growth factor stimulation, sufficient to maintain cell function while maximizing responsiveness to experimental stimuli. This low-growth-factor environment ensures that keratinocytes remain highly sensitive to external signals, making it ideal for studies requiring controlled conditions.
The formulation is completely free of animal or human-derived components and does not contain phenol red or antibiotics/antimycotics. In the absence of strong growth factor stimulation typically found in standard proliferation media, keratinocytes cultured in CnT-PR-H exhibit heightened responsiveness to experimental treatments. For example, responsiveness to Vitamin C (100 µg/mL) is five times higher than in standard proliferation medium. In this assay, confluent dermal fibroblasts were switched to the respective media and grown for five days (see image). Total collagen was then lysed in-well using acetic acid, quantified with Sirius Red, and normalized to gDNA to correct for cell number differences.